Haplotypes of The BoLA-A, Bf, Cyp21 and DQB LOCI in Angus Cattle

M. R. Teutsch, J. A. Stewart-Haynes, J. E. Beever, A. Xu, L. B. Schook, H. A. Lewin

Research output: Contribution to journalArticlepeer-review


The genetic diversity of bovine major histocompatibility complex (BoLA) haplotypes within the Angus breed was studied. Four BoLA loci were chosen for our study; BoLA-A, complement factor B (Bf), cytochrome P450 steroid 21-hydroxylase (CYP21) and BoLA-DQB. Polymorphism of BoLA-A products was determined by serology. Alleles of the Bf, CYP21 and BoLA-DQB loci were distinguished by restriction fragment length polymorphism (RFLP) analysis. Thirteen different haplotypes were identified, eleven of which were confirmed by segregation analysis in a paternal half-sibling family of Angus cattle (n=9 offspring). These thirteen haplotypes were comprised of 9 BoLA-A alleles and a “blank”, two Bf alleles detected with Taql, two CYP21 alleles detected with Pst I, and eight DQB alleles detected with TaqI and PvuII. Two haplotypes containing the supertypic BoLA-URAA specificity were clearly differentiated by Bf and DQB typing. Two haplotypes distinguished by the BoLA-A alleles w2 and w3, shared the same DQB*2 allele. Nine and four haplotypes carried the Bf*1and Bf*2 alleles, respectively. Three haplotypes carried CYP21*A and ten contained CYP21*B. Linkage between BoLA-A and the CYP21 locus, both previously mapped to chromosome 23 by synteny mapping, was confirmed by segregation analysis. These results demonstrate that extended BoLA haplotypes are useful for studying genetic diversity within a breed.

Original languageEnglish (US)
Pages (from-to)185-199
Number of pages15
JournalAnimal Biotechnology
Issue number2
StatePublished - Jan 1 1990

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Animal Science and Zoology


Dive into the research topics of 'Haplotypes of The BoLA-A, Bf, Cyp21 and DQB LOCI in Angus Cattle'. Together they form a unique fingerprint.

Cite this