Haplotype-resolved genome of Mimosa bimucronata revealed insights into leaf movement and nitrogen fixation

Haifeng Jia, Jishan Lin, Zhicong Lin, Yibin Wang, Liangwei Xu, Wenjie Ding, Ray Ming

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Mimosa bimucronata originates from tropical America and exhibits distinctive leaf movement characterized by a relative slow speed. Additionally, this species possesses the ability to fix nitrogen. Despite these intriguing traits, comprehensive studies have been hindered by the lack of genomic resources for M. bimucronata. Results: To unravel the intricacies of leaf movement and nitrogen fixation, we successfully assembled a high-quality, haplotype-resolved, reference genome at the chromosome level, spanning 648 Mb and anchored in 13 pseudochromosomes. A total of 32,146 protein-coding genes were annotated. In particular, haplotype A was annotated with 31,035 protein-coding genes, and haplotype B with 31,440 protein-coding genes. Structural variations (SVs) and allele specific expression (ASE) analyses uncovered the potential role of structural variants in leaf movement and nitrogen fixation in M. bimucronata. Two whole-genome duplication (WGD) events were detected, that occurred ~ 2.9 and ~ 73.5 million years ago. Transcriptome and co-expression network analyses revealed the involvement of aquaporins (AQPs) and Ca2+-related ion channel genes in leaf movement. Moreover, we also identified nodulation-related genes and analyzed the structure and evolution of the key gene NIN in the process of symbiotic nitrogen fixation (SNF). Conclusion: The detailed comparative genomic and transcriptomic analyses provided insights into the mechanisms governing leaf movement and nitrogen fixation in M. bimucronata. This research yielded genomic resources and provided an important reference for functional genomic studies of M. bimucronata and other legume species.

Original languageEnglish (US)
Article number334
JournalBMC genomics
Volume25
Issue number1
DOIs
StatePublished - Dec 2024
Externally publishedYes

Keywords

  • Genome assembly
  • Leaf movements
  • Mimosa bimucronata
  • Nitrogen fixation

ASJC Scopus subject areas

  • Genetics
  • Biotechnology

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