HAMr: A Mechanical Impactor for Repeated Dynamic Loading of In vitro Neuronal Networks

S. Koumlis, D. Buecker, G. Moler, V. Eliasson, P. Sengupta

Research output: Contribution to journalArticle

Abstract

A new tool for exploring the effects of repeated low-amplitude mechanical impacts onto in vitro neuronal networks is presented. The experimental setup, HAMr, is specifically designed to allow variability in impact conditions while ensuring a highly repeatable result. HAMr’s functionality to induce inflammation related to mild traumatic brain injuries has been validated by assessing its capability to induce elevated expression of the inflammatory protein IL-1 β in in vitro neuronal cell cultures. The two main results obtained for the inflammatory response in dissociated cortical networks, presented for a range of impact force amplitude and total number of impacts, can be summarized as follows. First, the results demonstrate a strong correlation between ensuing inflammation level and numbers of impact. Second, the results indicate a possible existence of a safe threshold of number of impacts that does not initiate an inflammatory response.

Original languageEnglish (US)
Pages (from-to)1441-1449
Number of pages9
JournalExperimental Mechanics
Volume55
Issue number8
DOIs
StatePublished - Oct 22 2015

Keywords

  • Blunt impact
  • Dynamic loading
  • In vitro
  • Neuronal networks
  • Repeated impacts

ASJC Scopus subject areas

  • Aerospace Engineering
  • Mechanics of Materials
  • Mechanical Engineering

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