Haemostatic factors in human peripheral afferent lymph

George J. Miller; David J. Howarth; Janet C. Attfield; C. Justin Cooke; M. Nazeem Nanjee; Waldemar L. Olszewski; James H. Morrissey; Norman E. Miller

Haemostatic factors in human peripheral afferent lymph

George J. Miller, David J. Howarth, Janet C. Attfield, C. Justin Cooke, M. Nazeem Nanjee, Waldemar L. Olszewski, James H. Morrissey, Norman E. Miller

Biochemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Peripheral afferent lymph was obtained by cannulation of a collecting vessel in 17 healthy men (mean age 26 years). Lymph/plasma ratios of all vitamin K-dependent factors were lower than expected from molecular weight. Factor VII, factor IX and tissue factor pathway inhibitor (TFPI) lymph/plasma activity ratios were higher than antigen ratios. Activated factor VII (FVIIa) and TFPI-Xa complex concentrations were higher in lymph than plasma, and the raised FVIIa did not appear to be due to cannulation. The fibrinogen lymph/plasma activity (Clauss) ratio averaged about 20% of the antigen ratio. The result of an ELISA for D-dimer was higher in lymph than plasma, often more than five-fold. This high level in lymph was not explored but may indicate proteolysis of fibrinogen and fibrin with release of D-like and D-dimer-like fragments in interstitial fluid.

Original language	English (US)
Pages (from-to)	427-432
Number of pages	6
Journal	Thrombosis and Haemostasis
Volume	83
Issue number	3
State	Published - Mar 25 2000

Keywords

D-dimer
Haemostatic factors
Peripheral lymph

ASJC Scopus subject areas

Hematology

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title = "Haemostatic factors in human peripheral afferent lymph",

abstract = "Peripheral afferent lymph was obtained by cannulation of a collecting vessel in 17 healthy men (mean age 26 years). Lymph/plasma ratios of all vitamin K-dependent factors were lower than expected from molecular weight. Factor VII, factor IX and tissue factor pathway inhibitor (TFPI) lymph/plasma activity ratios were higher than antigen ratios. Activated factor VII (FVIIa) and TFPI-Xa complex concentrations were higher in lymph than plasma, and the raised FVIIa did not appear to be due to cannulation. The fibrinogen lymph/plasma activity (Clauss) ratio averaged about 20% of the antigen ratio. The result of an ELISA for D-dimer was higher in lymph than plasma, often more than five-fold. This high level in lymph was not explored but may indicate proteolysis of fibrinogen and fibrin with release of D-like and D-dimer-like fragments in interstitial fluid.",

keywords = "D-dimer, Haemostatic factors, Peripheral lymph",

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language = "English (US)",

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AU - Miller, George J.

AU - Howarth, David J.

AU - Attfield, Janet C.

AU - Cooke, C. Justin

AU - Nanjee, M. Nazeem

AU - Olszewski, Waldemar L.

AU - Morrissey, James H.

AU - Miller, Norman E.

PY - 2000/3/25

Y1 - 2000/3/25

N2 - Peripheral afferent lymph was obtained by cannulation of a collecting vessel in 17 healthy men (mean age 26 years). Lymph/plasma ratios of all vitamin K-dependent factors were lower than expected from molecular weight. Factor VII, factor IX and tissue factor pathway inhibitor (TFPI) lymph/plasma activity ratios were higher than antigen ratios. Activated factor VII (FVIIa) and TFPI-Xa complex concentrations were higher in lymph than plasma, and the raised FVIIa did not appear to be due to cannulation. The fibrinogen lymph/plasma activity (Clauss) ratio averaged about 20% of the antigen ratio. The result of an ELISA for D-dimer was higher in lymph than plasma, often more than five-fold. This high level in lymph was not explored but may indicate proteolysis of fibrinogen and fibrin with release of D-like and D-dimer-like fragments in interstitial fluid.

AB - Peripheral afferent lymph was obtained by cannulation of a collecting vessel in 17 healthy men (mean age 26 years). Lymph/plasma ratios of all vitamin K-dependent factors were lower than expected from molecular weight. Factor VII, factor IX and tissue factor pathway inhibitor (TFPI) lymph/plasma activity ratios were higher than antigen ratios. Activated factor VII (FVIIa) and TFPI-Xa complex concentrations were higher in lymph than plasma, and the raised FVIIa did not appear to be due to cannulation. The fibrinogen lymph/plasma activity (Clauss) ratio averaged about 20% of the antigen ratio. The result of an ELISA for D-dimer was higher in lymph than plasma, often more than five-fold. This high level in lymph was not explored but may indicate proteolysis of fibrinogen and fibrin with release of D-like and D-dimer-like fragments in interstitial fluid.

KW - D-dimer

KW - Haemostatic factors

KW - Peripheral lymph

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SN - 0340-6245

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ER -

Haemostatic factors in human peripheral afferent lymph

Abstract

Keywords

ASJC Scopus subject areas

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