Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways

Hannah L. Klein, Giedrė Bačinskaja, Jun Che, Anais Cheblal, Rajula Elango, Anastasiya Epshtein, Devon M. Fitzgerald, Belén Gómez-González, Sharik R. Khan, Sandeep Kumar, Bryan A. Leland, Léa Marie, Qian Mei, Judith Miné-Hattab, Alicja Piotrowska, Erica J. Polleys, Christopher D. Putnam, Elina A. Radchenko, Anissia Ait Saada, Cynthia J. SakofskyEun Yong Shim, Mathew Stracy, Jun Xia, Zhenxin Yan, Yi Yin, Andrés Aguilera, Juan Lucas Argueso, Catherine H. Freudenreich, Susan M. Gasser, Dmitry A. Gordenin, James E. Haber, Grzegorz Ira, Sue Jinks-Robertson, Megan C. King, Richard D. Kolodner, Andrei Kuzminov, Sarah A.E. Lambert, Sang Eun Lee, Kyle M. Miller, Sergei M. Mirkin, Thomas D. Petes, Susan M. Rosenberg, Rodney Rothstein, Lorraine S. Symington, Pawel Zawadzki, Nayun Kim, Michael Lisby, Anna Malkova

Research output: Contribution to journalReview article

Abstract

Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic, molecular, and cytological reporters. The assays are powerful tools but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies.

Original languageEnglish (US)
Pages (from-to)1-64
Number of pages64
JournalMicrobial Cell
Volume6
Issue number1
DOIs
StatePublished - Jan 2019

Keywords

  • Chromatin dynamics
  • Chromosome rearrangements
  • Crossovers
  • DNA breaks
  • DNA repair centers
  • DNA resection
  • DSBs
  • Fluorescent proteins
  • Gene amplification
  • Gene conversion
  • Genome instability
  • Gross chromosome rearrangements
  • Holliday junctions
  • Homologous recombination
  • Mitotic recombination
  • Mutagenesis
  • Pulsed field gel electrophoresis
  • R-loops
  • Replication fork stalling
  • Single-particle tracking
  • Sister chromatid recombination
  • Sister repetitive sequences
  • Site-specific chromosome breaks
  • Toxic recombination intermediates
  • Yeast artificial chromosome

ASJC Scopus subject areas

  • Immunology and Microbiology (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

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  • Cite this

    Klein, H. L., Bačinskaja, G., Che, J., Cheblal, A., Elango, R., Epshtein, A., Fitzgerald, D. M., Gómez-González, B., Khan, S. R., Kumar, S., Leland, B. A., Marie, L., Mei, Q., Miné-Hattab, J., Piotrowska, A., Polleys, E. J., Putnam, C. D., Radchenko, E. A., Saada, A. A., ... Malkova, A. (2019). Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways. Microbial Cell, 6(1), 1-64. https://doi.org/10.15698/mic2019.01.664