TY - JOUR
T1 - Growth at elevated ozone or elevated carbon dioxide concentration alters antioxidant capacity and response to acute oxidative stress in soybean (Glycine max)
AU - Gillespie, Kelly M.
AU - Rogers, Alistair
AU - Ainsworth, Elizabeth A.
N1 - Funding Information:
We thank Andrew Leakey, Don Ort, Tim Mies, and Jesse McGrath for construction and support of the growth chamber facility. We also thank Robert Koester and Jessica Chiang for assistance in plant maintenance, tissue sampling, and biochemistry. Finally, we thank Craig Yendrek and Carrie Ramig for assistance with the real-time PCR. KMG was supported by a Graduate Research for the Environment Fellowship from the US Department of Energy’s Global Change Education Program. Funding for building the ozone fumigation system was provided by the Illinois Council for Food and Agricultural Research (CFAR). This research was supported in part by the US Department of Energy contract No. DE-AC02-98CH10886 to Brookhaven National Laboratory.
PY - 2011/5
Y1 - 2011/5
N2 - Soybeans (Glycine max Merr.) were grown at elevated carbon dioxide concentration ([CO2]) or chronic elevated ozone concentration ([O3]; 90ppb), and then exposed to an acute O3 stress (200ppb for 4h) in order to test the hypothesis that the atmospheric environment alters the total antioxidant capacity of plants, and their capacity to respond to an acute oxidative stress. Total antioxidant metabolism, antioxidant enzyme activity, and antioxidant transcript abundance were characterized before, immediately after, and during recovery from the acute O3 treatment. Growth at chronic elevated [O3] increased the total antioxidant capacity of plants, while growth at elevated [CO2] decreased the total antioxidant capacity. Changes in total antioxidant capacity were matched by changes in ascorbate content, but not phenolic content. The growth environment significantly altered the pattern of antioxidant transcript and enzyme response to the acute O3 stress. Following the acute oxidative stress, there was an immediate transcriptional reprogramming that allowed for maintained or increased antioxidant enzyme activities in plants grown at elevated [O3]. Growth at elevated [CO2] appeared to increase the response of antioxidant enzymes to acute oxidative stress, but dampened and delayed the transcriptional response. These results provide evidence that the growth environment alters the antioxidant system, the immediate response to an acute oxidative stress, and the timing over which plants return to initial antioxidant levels. The results also indicate that future elevated [CO2] and [O3] will differentially affect the antioxidant system.
AB - Soybeans (Glycine max Merr.) were grown at elevated carbon dioxide concentration ([CO2]) or chronic elevated ozone concentration ([O3]; 90ppb), and then exposed to an acute O3 stress (200ppb for 4h) in order to test the hypothesis that the atmospheric environment alters the total antioxidant capacity of plants, and their capacity to respond to an acute oxidative stress. Total antioxidant metabolism, antioxidant enzyme activity, and antioxidant transcript abundance were characterized before, immediately after, and during recovery from the acute O3 treatment. Growth at chronic elevated [O3] increased the total antioxidant capacity of plants, while growth at elevated [CO2] decreased the total antioxidant capacity. Changes in total antioxidant capacity were matched by changes in ascorbate content, but not phenolic content. The growth environment significantly altered the pattern of antioxidant transcript and enzyme response to the acute O3 stress. Following the acute oxidative stress, there was an immediate transcriptional reprogramming that allowed for maintained or increased antioxidant enzyme activities in plants grown at elevated [O3]. Growth at elevated [CO2] appeared to increase the response of antioxidant enzymes to acute oxidative stress, but dampened and delayed the transcriptional response. These results provide evidence that the growth environment alters the antioxidant system, the immediate response to an acute oxidative stress, and the timing over which plants return to initial antioxidant levels. The results also indicate that future elevated [CO2] and [O3] will differentially affect the antioxidant system.
KW - Antioxidant metabolism
KW - ascorbate
KW - dehydroascorbate reductase
KW - glutathione reductase
KW - oxidative stress
KW - ozone pollution
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U2 - 10.1093/jxb/erq435
DO - 10.1093/jxb/erq435
M3 - Article
C2 - 21282325
AN - SCOPUS:79957445241
SN - 0022-0957
VL - 62
SP - 2667
EP - 2678
JO - Journal of experimental botany
JF - Journal of experimental botany
IS - 8
ER -