Graphene Nanopores for Electronic Recognition of DNA Methylation

Aditya Sarathy, Hu Qiu, Jean Pierre Leburton

Research output: Contribution to journalArticlepeer-review

Abstract

We investigate theoretically the ability of graphene nanopore membranes to detect methylated sites along a DNA molecule by electronic sheet current along the two-dimensional (2D) materials. Special emphasis is placed on the detection sensitivity changes due to pore size, shape, position, and the presence of defects around the nanopore in a membrane with constricted geometry. Enhanced sensitivity for detecting methylated CpG sites, labeled by methyl-CpG binding domain (MBD) proteins along a DNA molecule, is obtained for electronic transport through graphene midgap states caused by the constriction. A large square deviation from the graphene conductance with respect to the open nanopore is observed during the translocation of MBD proteins. This approach exhibits superior resolution in the detection of multiple methylated sites along the DNA compared to conventional ionic current blockade techniques.

Original languageEnglish (US)
Pages (from-to)3757-3763
Number of pages7
JournalJournal of Physical Chemistry B
Volume121
Issue number15
DOIs
StatePublished - Apr 20 2017

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

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