TY - JOUR
T1 - Graphene Nanopores for Electronic Recognition of DNA Methylation
AU - Sarathy, Aditya
AU - Qiu, Hu
AU - Leburton, Jean Pierre
N1 - Publisher Copyright:
© 2016 American Chemical Society.
PY - 2017/4/20
Y1 - 2017/4/20
N2 - We investigate theoretically the ability of graphene nanopore membranes to detect methylated sites along a DNA molecule by electronic sheet current along the two-dimensional (2D) materials. Special emphasis is placed on the detection sensitivity changes due to pore size, shape, position, and the presence of defects around the nanopore in a membrane with constricted geometry. Enhanced sensitivity for detecting methylated CpG sites, labeled by methyl-CpG binding domain (MBD) proteins along a DNA molecule, is obtained for electronic transport through graphene midgap states caused by the constriction. A large square deviation from the graphene conductance with respect to the open nanopore is observed during the translocation of MBD proteins. This approach exhibits superior resolution in the detection of multiple methylated sites along the DNA compared to conventional ionic current blockade techniques.
AB - We investigate theoretically the ability of graphene nanopore membranes to detect methylated sites along a DNA molecule by electronic sheet current along the two-dimensional (2D) materials. Special emphasis is placed on the detection sensitivity changes due to pore size, shape, position, and the presence of defects around the nanopore in a membrane with constricted geometry. Enhanced sensitivity for detecting methylated CpG sites, labeled by methyl-CpG binding domain (MBD) proteins along a DNA molecule, is obtained for electronic transport through graphene midgap states caused by the constriction. A large square deviation from the graphene conductance with respect to the open nanopore is observed during the translocation of MBD proteins. This approach exhibits superior resolution in the detection of multiple methylated sites along the DNA compared to conventional ionic current blockade techniques.
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U2 - 10.1021/acs.jpcb.6b11040
DO - 10.1021/acs.jpcb.6b11040
M3 - Article
C2 - 28035832
AN - SCOPUS:85020190230
SN - 1520-6106
VL - 121
SP - 3757
EP - 3763
JO - Journal of Physical Chemistry B
JF - Journal of Physical Chemistry B
IS - 15
ER -