Gradient fi eld microscopy allows label-free disease diagnosis

Taewoo Kim, Shamira Sridharan, Gabriel Popescu

Research output: Contribution to journalArticlepeer-review


Quantitative Light Imaging (QLI) Laboratory has developed a Gradient field microscopy (GFM) method that provides high-contrast images of transparent specimens, including cells and tissues. In GFM, the spatial change of the optical path length is measured by taking the first or second order derivatives of the phase information that is carried by the light. The aperture stop is closed down for the highest possible spatial coherence of the illumination. The system consists of a series of two lenses forming a 4f system to provide access to the Fourier transform of the image field. At this Fourier plane, spatial amplitude modulation is provided through a spatial light modulator. One exciting feature of the GFM is that it can image HGPIN condition in which the epithelial cells of the prostate glands have morphological characteristics associated with prostate cancer such as prominent nucleoli and Roman bridge formation. The GFM setup has fast acquisition speed and stability along with the flexibility of using the different modes of operation.

Original languageEnglish
Pages (from-to)41-44
Number of pages4
JournalLaser Focus World
Issue number8
StatePublished - Aug 1 2012

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Atomic and Molecular Physics, and Optics
  • Electrical and Electronic Engineering
  • Marketing


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