Glycogen synthesis by cell-free extracts from the dimorphic yeast Candida albicans

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Abstract

A particulate glucosyltransferase prepared from budding and filamentous cultures of Candida albicans used uridine diphosphate glucose as sole glucosyl donor in a reaction (measured by following the incorporation of [14C]-glucose from UDP [14C]-glucose into polymer) stimulated by glucose-6-phosphate and inhibited by adenosine triphosphate and guanosine triphosphate. The radiolabelled reaction product was solubilized by α-amylase, and, on oxidation with periodate followed by reduction with borohydride and acid hydrolysis, yielded erythritol and glycerol in the ratio of 4 to 1. The radiolabelled glucosyl residues were attached to an endogenous acceptor of high molecular weight.

Original languageEnglish (US)
Pages (from-to)341-348
Number of pages8
JournalAntonie van Leeuwenhoek
Volume50
Issue number4
DOIs
StatePublished - Jul 1 1984
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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