Glycerol permeability of rye leaf protoplasts as affected by temperature and electroporation

R. E. Pitt, J. E. Parks, S. C. Huber, J. A. Sangree

Research output: Contribution to journalArticle

Abstract

The permeability of rye leaf protoplasts to glycerol was determined using 1,3-14C glycerol and liquid scintillation spectrometry. Estimates were 1.0 x 10-8 m s-1 at 0 °C and 4.1 x 10-8 m s-1 at 22 and 31 °C. The activation energy for glycerol permeability was 32.8 kJ/mol. The effect of electroporation on glycerol uptake was also explored. Treatments were performed with a field strength of 100 V/cm and an exponential decay constant of 5.8 ms. At 22 °C, electroporation affected the rate and extent of glycerol permeation, causing an increase in the intercept of the glycerol uptake curve and a decrease in the slope. Electroporation had no significant effect on glycerol uptake when performed at 0 °C, when the cells were electroporated at 0 °C then warmed to 31 °C, or when the cells were electroporated at 22 °C then cooled to 0 °C. The results at 22 °C were consistent with an influx of glycerol during electroporation.

Original languageEnglish (US)
Pages (from-to)215-219
Number of pages5
JournalPlant Cell, Tissue and Organ Culture
Volume50
Issue number3
DOIs
StatePublished - Dec 1 1997

Keywords

  • Activation energy
  • Electroporation
  • Glycerol
  • Permeability
  • Secale cereale

ASJC Scopus subject areas

  • Horticulture

Fingerprint Dive into the research topics of 'Glycerol permeability of rye leaf protoplasts as affected by temperature and electroporation'. Together they form a unique fingerprint.

  • Cite this