TY - JOUR
T1 - Glutathione Utilization in Lactobacillus fermentum CECT 5716
AU - Surya, Agustian
AU - Liu, Xiaoji
AU - Miller, Michael J.
N1 - We are especially thankful for the Fulbright Scholarship, administered by Institute of International Education, that supported AS. This research and XL were supported by USDA-NIFA (Grant No. 2016-67017-24430).
*Phone: (217) 244-1973; Fax: (217) 265-0925; E-mail: [email protected]. ORCID Agustian Surya: 0000-0002-4706-0700 Michael J. Miller: 0000-0001-8579-1080 Funding We are especially thankful for the Fulbright Scholarship, administered by Institute of International Education, that supported AS. This research and XL were supported by USDA-NIFA (Grant No. 2016-67017-24430). Notes The authors declare no competing financial interest.
PY - 2018/12/5
Y1 - 2018/12/5
N2 - Glutathione, a tripeptide antioxidant, has recently been shown to be either utilized or synthesized by Gram-positive bacteria, such as lactic acid bacteria. Glutathione plays an important role in countering environmental stress, such as oxidative stress. In this study, cellular activity regarding glutathione in Lactobacillus fermentum CECT 5716 is characterized. We demonstrate that L. fermentum CECT 5716 has a better survival rate in the presence of glutathione under both oxidative and metal stress. As L. fermentum CECT 5716 does not possess the ability to synthesize glutathione under the conditions tested, it shows the ability to uptake both reduced and oxidized glutathione from the environment, regenerate reduced glutathione from oxidized glutathione, and perform secretion of glutathione to the environment.
AB - Glutathione, a tripeptide antioxidant, has recently been shown to be either utilized or synthesized by Gram-positive bacteria, such as lactic acid bacteria. Glutathione plays an important role in countering environmental stress, such as oxidative stress. In this study, cellular activity regarding glutathione in Lactobacillus fermentum CECT 5716 is characterized. We demonstrate that L. fermentum CECT 5716 has a better survival rate in the presence of glutathione under both oxidative and metal stress. As L. fermentum CECT 5716 does not possess the ability to synthesize glutathione under the conditions tested, it shows the ability to uptake both reduced and oxidized glutathione from the environment, regenerate reduced glutathione from oxidized glutathione, and perform secretion of glutathione to the environment.
KW - Lactobacillus fermentum
KW - glutathione
KW - lactic acid bacteria
KW - metal stress
KW - oxidative stress
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U2 - 10.1021/acs.jafc.8b06136
DO - 10.1021/acs.jafc.8b06136
M3 - Article
C2 - 30417643
AN - SCOPUS:85057859222
SN - 0021-8561
VL - 66
SP - 12651
EP - 12656
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 48
ER -