Glutathione and bile acid synthesis. Effect of GSH content of HepG2 cells on the activity and mRNA levels of cholesterol 7α-hydroxylase

Aslam S. Hassan; David Bunick; Lisa A. Lund; Walter G. Bottje

doi:10.1016/0006-2952(92)90553-U

Glutathione and bile acid synthesis. Effect of GSH content of HepG2 cells on the activity and mRNA levels of cholesterol 7α-hydroxylase

Aslam S. Hassan, David Bunick, Lisa A. Lund, Walter G. Bottje

Comparative Biosciences

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Abstract

Cholesterol 7α-hydroxylase (CH-7α) activity in HepG2 cells depleted of glutathione (GSH) was reduced significantly (P < 0.05) compared to that in untreated controls. Northern blot analysis of poly A⁺ mRNA isolated from GSH-depleted and control HepG2 cells showed that there was a reduction in mRNA for CH-7α in treated HepG2 cells that was commensurate with the reduction in CH-7α activity. The fact that total RNA, rRNA, and mRNA for β fibrinogen were unaltered by the depletion of GSH suggests that the change in steady-state CH-7α mRNA content is specifically sensitive to GSH content. This observation represents the first demonstration, for human liver cells, that there is an interaction between GSH levels and the regulation of CH-7α mRNA levels.

Original language	English (US)
Pages (from-to)	1475-1477
Number of pages	3
Journal	Biochemical Pharmacology
Volume	44
Issue number	7
DOIs	https://doi.org/10.1016/0006-2952(92)90553-U
State	Published - Oct 6 1992

ASJC Scopus subject areas

Biochemistry
Pharmacology

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10.1016/0006-2952(92)90553-U

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@article{4e77851bb1404ab2861d1b2611a0ae93,

title = "Glutathione and bile acid synthesis. Effect of GSH content of HepG2 cells on the activity and mRNA levels of cholesterol 7α-hydroxylase",

abstract = "Cholesterol 7α-hydroxylase (CH-7α) activity in HepG2 cells depleted of glutathione (GSH) was reduced significantly (P < 0.05) compared to that in untreated controls. Northern blot analysis of poly A+ mRNA isolated from GSH-depleted and control HepG2 cells showed that there was a reduction in mRNA for CH-7α in treated HepG2 cells that was commensurate with the reduction in CH-7α activity. The fact that total RNA, rRNA, and mRNA for β fibrinogen were unaltered by the depletion of GSH suggests that the change in steady-state CH-7α mRNA content is specifically sensitive to GSH content. This observation represents the first demonstration, for human liver cells, that there is an interaction between GSH levels and the regulation of CH-7α mRNA levels.",

author = "Hassan, {Aslam S.} and David Bunick and Lund, {Lisa A.} and Bottje, {Walter G.}",

note = "Funding Information: Acknowledgements-The authorsa re gratefult o Dr. David Russell,U niversityo f TexasS outhwesternM edicalC enter, for providingt he cDNA of rat CH-7a and to Dr. Samar N. Roy, Lindlsey F. Kimball ResearchI nstitute,f or the /3 fibrinogenp robe. This studyw as supported,i n part, by a grant from the American Heart Association, Illinois Affiliate.",

year = "1992",

month = oct,

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doi = "10.1016/0006-2952(92)90553-U",

language = "English (US)",

volume = "44",

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journal = "Biochemical Pharmacology",

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publisher = "Elsevier Inc.",

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TY - JOUR

T1 - Glutathione and bile acid synthesis. Effect of GSH content of HepG2 cells on the activity and mRNA levels of cholesterol 7α-hydroxylase

AU - Hassan, Aslam S.

AU - Bunick, David

AU - Lund, Lisa A.

AU - Bottje, Walter G.

N1 - Funding Information: Acknowledgements-The authorsa re gratefult o Dr. David Russell,U niversityo f TexasS outhwesternM edicalC enter, for providingt he cDNA of rat CH-7a and to Dr. Samar N. Roy, Lindlsey F. Kimball ResearchI nstitute,f or the /3 fibrinogenp robe. This studyw as supported,i n part, by a grant from the American Heart Association, Illinois Affiliate.

PY - 1992/10/6

Y1 - 1992/10/6

N2 - Cholesterol 7α-hydroxylase (CH-7α) activity in HepG2 cells depleted of glutathione (GSH) was reduced significantly (P < 0.05) compared to that in untreated controls. Northern blot analysis of poly A+ mRNA isolated from GSH-depleted and control HepG2 cells showed that there was a reduction in mRNA for CH-7α in treated HepG2 cells that was commensurate with the reduction in CH-7α activity. The fact that total RNA, rRNA, and mRNA for β fibrinogen were unaltered by the depletion of GSH suggests that the change in steady-state CH-7α mRNA content is specifically sensitive to GSH content. This observation represents the first demonstration, for human liver cells, that there is an interaction between GSH levels and the regulation of CH-7α mRNA levels.

AB - Cholesterol 7α-hydroxylase (CH-7α) activity in HepG2 cells depleted of glutathione (GSH) was reduced significantly (P < 0.05) compared to that in untreated controls. Northern blot analysis of poly A+ mRNA isolated from GSH-depleted and control HepG2 cells showed that there was a reduction in mRNA for CH-7α in treated HepG2 cells that was commensurate with the reduction in CH-7α activity. The fact that total RNA, rRNA, and mRNA for β fibrinogen were unaltered by the depletion of GSH suggests that the change in steady-state CH-7α mRNA content is specifically sensitive to GSH content. This observation represents the first demonstration, for human liver cells, that there is an interaction between GSH levels and the regulation of CH-7α mRNA levels.

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Glutathione and bile acid synthesis. Effect of GSH content of HepG2 cells on the activity and mRNA levels of cholesterol 7α-hydroxylase

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