Glutaredoxin AtGRXC2 catalyses inhibitory glutathionylation of arabidopsis BRI1-associated receptor-like kinase 1 (BAK1) in vitro

Kyle W. Bender, Xuejun Wang, George B. Cheng, Hyoung Seok Kim, Raymond E Zielinski, Steven C Huber

Research output: Contribution to journalArticle

Abstract

Reversible protein phosphorylation, catalysed by protein kinases, is the most widely studied post-translational modification (PTM), whereas the analysis of other modifications such as S-thiolation is in its relative infancy. In a yeast-two-hybrid (Y2H) screen, we identified a number of novel putative brassinosteroid insensitive 1 (BR1)-associated receptor-like kinase 1 (BAK1) interacting proteins including several proteins related to redox regulation. Glutaredoxin (GRX) C2 (AtGRXC2) was among candidate proteins identified in the Y2H screen and its interaction with recombinant Flag-BAK1 cytoplasmic domain was confirmed using an in vitro pull-down approach. We show that BAK1 peptide kinase activity is sensitive to the oxidizing agents H2O2 and diamide in vitro, suggesting that cysteine oxidation might contribute to control of BAK1 activity. Furthermore, BAK1 was glutathionylated and this reaction could occur via a thiolatedependent reactionwith GSSG or aH2O2-dependent reactionwith GSH and inhibited kinase activity. Surprisingly, both reactions were catalysed by AtGRXC2 at lower concentrations of GSSG or GSH than reacted non-enzymatically. Using MALDI-TOF MS, we identified Cys353, Cys374 and Cys408 as potential sites of glutathionylation on the BAK1 cytoplasmic domain and directed mutagenesis suggests that Cys353 and Cys408 are major sites of GRXC2-mediated glutathionylation. Collectively, these results highlight the potential for redox control of BAK1 and demonstrate the ability of AtGRXC2 to catalyse protein glutathionylation, a function not previously described for any plant GRX. The present work presents a foundation for future studies of glutathionylation of plant receptor-like protein kinases (RLKs) as well as for the analysis of activities of plant GRXs.

Original languageEnglish (US)
Pages (from-to)399-413
Number of pages15
JournalBiochemical Journal
Volume467
Issue number3
DOIs
StatePublished - May 1 2015

Fingerprint

Glutaredoxins
Arabidopsis
Phosphotransferases
Glutathione Disulfide
Proteins
Protein Kinases
Oxidation-Reduction
Brassinosteroids
Diamide
In Vitro Techniques
Mutagenesis
Phosphorylation
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Post Translational Protein Processing
Oxidants
Yeast
Cysteine
Yeasts

Keywords

  • Brassinosteroid insensitive 1-associated receptor-like kinase 1 (BAK1)
  • Glutaredoxin
  • Glutathionylation
  • Receptor-like kinase
  • Redox regulation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Glutaredoxin AtGRXC2 catalyses inhibitory glutathionylation of arabidopsis BRI1-associated receptor-like kinase 1 (BAK1) in vitro. / Bender, Kyle W.; Wang, Xuejun; Cheng, George B.; Kim, Hyoung Seok; Zielinski, Raymond E; Huber, Steven C.

In: Biochemical Journal, Vol. 467, No. 3, 01.05.2015, p. 399-413.

Research output: Contribution to journalArticle

Bender, Kyle W. ; Wang, Xuejun ; Cheng, George B. ; Kim, Hyoung Seok ; Zielinski, Raymond E ; Huber, Steven C. / Glutaredoxin AtGRXC2 catalyses inhibitory glutathionylation of arabidopsis BRI1-associated receptor-like kinase 1 (BAK1) in vitro. In: Biochemical Journal. 2015 ; Vol. 467, No. 3. pp. 399-413.
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AU - Wang, Xuejun

AU - Cheng, George B.

AU - Kim, Hyoung Seok

AU - Zielinski, Raymond E

AU - Huber, Steven C

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AB - Reversible protein phosphorylation, catalysed by protein kinases, is the most widely studied post-translational modification (PTM), whereas the analysis of other modifications such as S-thiolation is in its relative infancy. In a yeast-two-hybrid (Y2H) screen, we identified a number of novel putative brassinosteroid insensitive 1 (BR1)-associated receptor-like kinase 1 (BAK1) interacting proteins including several proteins related to redox regulation. Glutaredoxin (GRX) C2 (AtGRXC2) was among candidate proteins identified in the Y2H screen and its interaction with recombinant Flag-BAK1 cytoplasmic domain was confirmed using an in vitro pull-down approach. We show that BAK1 peptide kinase activity is sensitive to the oxidizing agents H2O2 and diamide in vitro, suggesting that cysteine oxidation might contribute to control of BAK1 activity. Furthermore, BAK1 was glutathionylated and this reaction could occur via a thiolatedependent reactionwith GSSG or aH2O2-dependent reactionwith GSH and inhibited kinase activity. Surprisingly, both reactions were catalysed by AtGRXC2 at lower concentrations of GSSG or GSH than reacted non-enzymatically. Using MALDI-TOF MS, we identified Cys353, Cys374 and Cys408 as potential sites of glutathionylation on the BAK1 cytoplasmic domain and directed mutagenesis suggests that Cys353 and Cys408 are major sites of GRXC2-mediated glutathionylation. Collectively, these results highlight the potential for redox control of BAK1 and demonstrate the ability of AtGRXC2 to catalyse protein glutathionylation, a function not previously described for any plant GRX. The present work presents a foundation for future studies of glutathionylation of plant receptor-like protein kinases (RLKs) as well as for the analysis of activities of plant GRXs.

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