Globin-coupled sensors: A class of heme-containing sensors in Archaea and Bacteria

Shaobin Hou, Tracey Freitas, Randy W. Larsen, Mikhail Piatibratov, Victor Sivozhelezov, Amy Yamamoto, Ella A. Meleshkevitch, Mike Zimmer, George W. Ordal, Maqsudul Alam

Research output: Contribution to journalArticle

Abstract

The recently discovered prokaryotic signal transducer HemAT, which has been described in both Archaea and Bacteria, mediates aerotactic responses. The N-terminal regions of HemAT from the archaeon Halobacterium salinarum (HemAT-Hs) and from the Gram-positive bacterium Bacillus subtilis (HemAT-Bs) contain a myoglobin-like motif, display characteristic heme-protein absorption spectra, and bind oxygen reversibly. Recombinant HemAT-Hs and HemAT-Bs shorter than 195 and 176 residues, respectively, do not bind heme effectively. Sequence homology comparisons and three-dimensional modeling predict that His-123 is the proximal heme-binding residue in HemAT from both species. The work described here used site-specific mutagenesis and spectroscopy to confirm this prediction, thereby providing direct evidence for a functional domain of prokaryotic signal transducers that bind heme in a globin fold. We postulate that this domain is part of a globin-coupled sensor (GCS) motif that exists as a two-domain transducer having no similarity to the PER-ARNT-SIM (PAS)-domain superfamily transducers. Using the GCS motif, we have identified several two-domain sensors in a variety of prokaryotes. We have cloned, expressed, and purified two potential globin-coupled sensors and performed spectral analysis on them. Both bind heme and show myoglobin-like spectra. This observation suggests that the general function of GCS-type transducers is to bind diatomic oxygen and perhaps other gaseous ligands, and to transmit a conformational signal through a linked signaling domain.

Original languageEnglish (US)
Pages (from-to)9353-9358
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number16
DOIs
StatePublished - Jul 31 2001

Keywords

  • Proximal histidine
  • Transducer

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Globin-coupled sensors: A class of heme-containing sensors in Archaea and Bacteria'. Together they form a unique fingerprint.

  • Cite this

    Hou, S., Freitas, T., Larsen, R. W., Piatibratov, M., Sivozhelezov, V., Yamamoto, A., Meleshkevitch, E. A., Zimmer, M., Ordal, G. W., & Alam, M. (2001). Globin-coupled sensors: A class of heme-containing sensors in Archaea and Bacteria. Proceedings of the National Academy of Sciences of the United States of America, 98(16), 9353-9358. https://doi.org/10.1073/pnas.161185598