Global Functional Map of the p23 Molecular Chaperone Reveals an Extensive Cellular Network

Frank J. Echtenkamp, Elena Zelin, Ellinor Oxelmark, Joyce I. Woo, Brenda J. Andrews, Michael Garabedian, Brian C. Freeman

Research output: Contribution to journalArticle

Abstract

In parallel with evolutionary developments, the Hsp90 molecular chaperone system shifted from a simple prokaryotic factor into an expansive network that includes a variety of cochaperones. We have taken high-throughput genomic and proteomic approaches to better understand the abundant yeast p23 cochaperone Sba1. Our work revealed an unexpected p23 network that displayed considerable independence from known Hsp90 clients. Additionally, our data uncovered a broad nuclear role for p23, contrasting with the historical dogma of restricted cytosolic activities for molecular chaperones. Validation studies demonstrated that yeast p23 was required for proper Golgi function and ribosome biogenesis, and was necessary for efficient DNA repair from a wide range of mutagens. Notably, mammalian p23 had conserved roles in these pathways as well as being necessary for proper cell mobility. Taken together, our work demonstrates that the p23 chaperone serves a broad physiological network and functions both in conjunction with and sovereign to Hsp90.

Original languageEnglish (US)
Pages (from-to)229-241
Number of pages13
JournalMolecular cell
Volume43
Issue number2
DOIs
StatePublished - Jul 22 2011

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Molecular Chaperones
Yeasts
Validation Studies
Mutagens
Ribosomes
DNA Repair
Proteomics

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Echtenkamp, F. J., Zelin, E., Oxelmark, E., Woo, J. I., Andrews, B. J., Garabedian, M., & Freeman, B. C. (2011). Global Functional Map of the p23 Molecular Chaperone Reveals an Extensive Cellular Network. Molecular cell, 43(2), 229-241. https://doi.org/10.1016/j.molcel.2011.05.029

Global Functional Map of the p23 Molecular Chaperone Reveals an Extensive Cellular Network. / Echtenkamp, Frank J.; Zelin, Elena; Oxelmark, Ellinor; Woo, Joyce I.; Andrews, Brenda J.; Garabedian, Michael; Freeman, Brian C.

In: Molecular cell, Vol. 43, No. 2, 22.07.2011, p. 229-241.

Research output: Contribution to journalArticle

Echtenkamp, FJ, Zelin, E, Oxelmark, E, Woo, JI, Andrews, BJ, Garabedian, M & Freeman, BC 2011, 'Global Functional Map of the p23 Molecular Chaperone Reveals an Extensive Cellular Network', Molecular cell, vol. 43, no. 2, pp. 229-241. https://doi.org/10.1016/j.molcel.2011.05.029
Echtenkamp FJ, Zelin E, Oxelmark E, Woo JI, Andrews BJ, Garabedian M et al. Global Functional Map of the p23 Molecular Chaperone Reveals an Extensive Cellular Network. Molecular cell. 2011 Jul 22;43(2):229-241. https://doi.org/10.1016/j.molcel.2011.05.029
Echtenkamp, Frank J. ; Zelin, Elena ; Oxelmark, Ellinor ; Woo, Joyce I. ; Andrews, Brenda J. ; Garabedian, Michael ; Freeman, Brian C. / Global Functional Map of the p23 Molecular Chaperone Reveals an Extensive Cellular Network. In: Molecular cell. 2011 ; Vol. 43, No. 2. pp. 229-241.
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