Germline translocations in mice: Unique tools for analyzing gene function and long-distance regulatory mechanisms

Colleen Elso, Xiaochen Lu, Stephanie Morrison, Angela Tarver, Heather Thompson, Hillary Thurkow, N. Alice Yamada, Lisa Stubbs

Research output: Contribution to journalArticlepeer-review

Abstract

Translocations have provided invaluable tools for identifying both cancer-linked genes and loci associated with heritable human diseases, but heritable human translocations are rare and few mouse models exist. Here we report progress on analysis of a collection of heritable translocations generated by treatment of mice with specific chemicals or radiation during late spermatogenic stages. The translocation mutants exhibit a range of visible phenotypes reflecting the disruption of coding sequences or the separation of genes from essential regulatory elements. The breakpoints of both radiation-induced and chemically induced mutations in these mice are remarkably clean, with very short deletions, duplications, or inversions in some cases, and ligation mediated by microhomology, suggesting nonhomologous end joining as the major path of repair. These mutations provide new tools for the discovery of novel genes and regulatory elements linked to human developmental disorders and new clues to the molecular basis of human genetic disease.

Original languageEnglish (US)
Pages (from-to)91-95
Number of pages5
JournalJournal of the National Cancer Institute - Monographs
Issue number39
DOIs
StatePublished - 2008
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Germline translocations in mice: Unique tools for analyzing gene function and long-distance regulatory mechanisms'. Together they form a unique fingerprint.

Cite this