Genotyping single nucleotide polymorphisms (SNPs) across species in Old World Monkeys

Ripan S. Malhi, Jessica Satkoski Trask, Milena Shattuck, Jesse Johnson, Debrapriyo Chakraborty, Sree Kanthaswamy, Uma Ramakrishnan, David Glenn Smith

Research output: Contribution to journalArticlepeer-review


The development of DNA markers is becoming increasingly useful in the field of primatology for studies on paternity, population history, and biomedical research. In this study, we determine the efficacy of using cross-species amplification to identify single nucleotide polymorphisms (SNPs) in closely related species. The DNA of 93 individuals representing seven Old World Monkey species was analyzed to identify SNPs using cross-species amplification and genotyping. The loci genotyped were 653 SNPs identified and validated in rhesus macaques. Of the 653 loci analyzed, 27% were estimated to be polymorphic in the samples studied. SNPs identified at the same locus among different species (coincident SNPs) were found in six of the seven species studied with longtail macaques exhibiting the highest number of coincident SNPs (84). The distribution of coincident SNPs among species is not biased based on proximity to genes in the samples studied. In addition, the frequency of coincident SNPs is not consistent with expectations based on their phylogenetic relationships. This study demonstrates that cross-species amplification and genotyping using the Illumina Golden Gate Array is a useful method to identify a large number of SNPs in closely related species, although issues with ascertainment bias may limit the type of studies where this method can be applied.

Original languageEnglish (US)
Pages (from-to)1031-1040
Number of pages10
JournalAmerican journal of primatology
Issue number10
StatePublished - Oct 2011


  • Coincident SNP
  • Cross-species amplification
  • DNA

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Animal Science and Zoology


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