Genotyping-by-Sequencing for Analysis of the Genetic Variation of Podosphaera xanthii, Incitant of Cucurbit Powdery Mildew

Y. Xiang, A. N. Miller, M. McGrath, M. Babadoost

Research output: Contribution to journalArticlepeer-review

Abstract

This research was conducted to identify species causing powdery mildew on cucurbits and to determine genetic variations among isolates of the pathogen. We collected 109 isolates from six cucurbit species hosts (Cucumis melo, Cucumis sativus, Cucurbita maxima, Cucurbita moschata, Cucurbita pepo, and Lagenaria siceraria) in California, Illinois, Indiana, Michigan, New York, Texas, Washington, and Wisconsin in the United States and in Italy. By sequencing the internal transcribed spacer region of the nuclear rDNA of these 109 isolates, Podosphaera xanthii was found as the only species causing powdery mildew on cucurbits in the United States. Genotyping-by-sequencing was applied to these 109 isolates to investigate their genetic diversity, which showed a trend of isolates clustering from New York and Italy. In addition, the virulence of 36 isolates was compared and a significant difference (P < 0.0001) was found among them. Furthermore, results of the virulence tests of 28 isolates from Illinois showed significant effects of collection years, hosts, and locations on the virulence of the isolates.

Original languageEnglish (US)
Pages (from-to)951-957
Number of pages7
JournalPlant disease
Volume104
Issue number3
DOIs
StatePublished - Jan 1 2020

Keywords

  • Cucurbits
  • Genetic variation
  • Podosphaera xanthii
  • Powdery mildew
  • Virulence variation
  • INHS

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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