Genomic, Transcriptional, and Phenotypic Analysis of the Glucose Derepressed Clostridium beijerinckii Mutant Exhibiting Acid Crash Phenotype

Seung Oh Seo, Holger Janssen, Andrew Magis, Yi Wang, Ting Lu, Nathan D. Price, Yong-Su Jin, Hans-Peter M Blaschek

Research output: Contribution to journalArticle

Abstract

Clostridium beijerinckii is a predominant solventogenic bacterium that is used for the ABE fermentation. Various C. beijerinckii mutants are constructed for desirable phenotypes. The C. beijerinckii mutant BA105 harboring a glucose derepression phenotype was previously isolated and demonstrated the enhanced amylolytic activity in the presence of glucose. Despite its potential use, BA105 is not further characterized and utilized. Therefore, the authors investigate fermentation phenotypes of BA105 in this study. Under the typical batch fermentation conditions, BA105 consistently exhibits acid crash phenotype resulting in limited glucose uptake and cell growth. However, when the culture pH is maintained above 5.5, BA105 exhibits the increased glucose uptake and butanol production than did the wild-type. To further analyze BA105, the authors perform genome sequencing and RNA sequencing. Genome analysis identifies two SNPs unique to BA105, in the upstream region of AbrB regulator (Cbei_4885) and the ROK family glucokinase (Cbei_4895) which are involved in catabolite repression and regulation of sugar metabolism. Transcriptional analysis of BA105 reveals significant differential expression of the genes associated with the PTS sugar transport system and acid production. This study improves understanding of the acid crash phenomenon and provides the genetic basis underlying the catabolite derepression phenotype of C. beijericnkii.

Original languageEnglish (US)
Article number1700182
JournalBiotechnology Journal
Volume12
Issue number11
DOIs
StatePublished - Nov 2017

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Clostridium beijerinckii
Phenotype
Glucose
Acids
Fermentation
Genetic Phenomena
Genome
Catabolite Repression
RNA Sequence Analysis
Glucokinase
Butanols
Single Nucleotide Polymorphism
Bacteria
Gene Expression
Growth

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Molecular Medicine

Cite this

Genomic, Transcriptional, and Phenotypic Analysis of the Glucose Derepressed Clostridium beijerinckii Mutant Exhibiting Acid Crash Phenotype. / Seo, Seung Oh; Janssen, Holger; Magis, Andrew; Wang, Yi; Lu, Ting; Price, Nathan D.; Jin, Yong-Su; Blaschek, Hans-Peter M.

In: Biotechnology Journal, Vol. 12, No. 11, 1700182, 11.2017.

Research output: Contribution to journalArticle

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