Genomic selection in wheat breeding using genotyping-by-sequencing

Jesse Poland, Jeffrey Endelman, Julie Dawson, Jessica Rutkoski, Shuangye Wu, Yann Manes, Susanne Dreisigacker, José Crossa, Héctor Sánchez-Villeda, Mark Sorrells, Jean Luc Jannink

Research output: Contribution to journalArticle

Abstract

Genomic selection (GS) uses genomewide molecular markers to predict breeding values and make selections of individuals or breeding lines prior to phenotyping. Here we show that genotyping-by-sequencing (GBS) can be used for de novo genotyping of breeding panels and to develop accurate GS models, even for the large, complex, and polyploid wheat (Triticum aestivum L.) genome. WiThGBS we discovered 41,371 single nucleotide polymorphisms (SNPs) in a set of 254 advanced breeding lines from CIMMYT's semiarid wheat breeding program. Four different methods were evaluated for imputing missing marker scores in this set of unmapped markers, including random forest regression and a newly developed multivariate-normal expectation-maximization algorithm, which gave more accurate imputation than heterozygous or mean imputation at the marker level, although no signifi cant differences were observed in the accuracy of genomic-estimated breeding values (GEBVs) among imputation methods. Genomic-estimated breeding value prediction accuracies wiThGBS were 0.28 to 0.45 for grain yield, an improvement of 0.1 to 0.2 over an established marker platform for wheat. Genotyping-bysequencing combines marker discovery and genotyping of large populations, making it an excellent marker platform for breeding applications even in the absence of a reference genome sequence or previous polymorphism discovery. In addition, the flexibility and low cost of GBS make this an ideal approach for genomics-assisted breeding.

Original languageEnglish (US)
Pages (from-to)103-113
Number of pages11
JournalPlant Genome
Volume5
Issue number3
DOIs
StatePublished - Dec 1 2012
Externally publishedYes

Fingerprint

marker-assisted selection
genotyping
Triticum
Breeding
breeding value
wheat
breeding
breeding lines
genomics
genome
polyploidy
single nucleotide polymorphism
Genome
Triticum aestivum
grain yield
genetic polymorphism
Polyploidy
phenotype
genetic markers
prediction

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

Cite this

Poland, J., Endelman, J., Dawson, J., Rutkoski, J., Wu, S., Manes, Y., ... Jannink, J. L. (2012). Genomic selection in wheat breeding using genotyping-by-sequencing. Plant Genome, 5(3), 103-113. https://doi.org/10.3835/plantgenome2012.06.0006

Genomic selection in wheat breeding using genotyping-by-sequencing. / Poland, Jesse; Endelman, Jeffrey; Dawson, Julie; Rutkoski, Jessica; Wu, Shuangye; Manes, Yann; Dreisigacker, Susanne; Crossa, José; Sánchez-Villeda, Héctor; Sorrells, Mark; Jannink, Jean Luc.

In: Plant Genome, Vol. 5, No. 3, 01.12.2012, p. 103-113.

Research output: Contribution to journalArticle

Poland, J, Endelman, J, Dawson, J, Rutkoski, J, Wu, S, Manes, Y, Dreisigacker, S, Crossa, J, Sánchez-Villeda, H, Sorrells, M & Jannink, JL 2012, 'Genomic selection in wheat breeding using genotyping-by-sequencing', Plant Genome, vol. 5, no. 3, pp. 103-113. https://doi.org/10.3835/plantgenome2012.06.0006
Poland, Jesse ; Endelman, Jeffrey ; Dawson, Julie ; Rutkoski, Jessica ; Wu, Shuangye ; Manes, Yann ; Dreisigacker, Susanne ; Crossa, José ; Sánchez-Villeda, Héctor ; Sorrells, Mark ; Jannink, Jean Luc. / Genomic selection in wheat breeding using genotyping-by-sequencing. In: Plant Genome. 2012 ; Vol. 5, No. 3. pp. 103-113.
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