Genomic organization, expression, and analysis of the troponin C gene pat-10 of Caenorhabditis elegans

Hiromi Terami, Benjamin D. Williams, Shin Ichi Kitamura, Yasuji Sakube, Shinji Matsumoto, Shima Doi, Takashi Obinata, Hiroaki Kagawa

    Research output: Contribution to journalArticlepeer-review


    We have cloned and characterized the troponin C gene, pat-10 of the nematode Caenorhabditis elegans. At the amino acid level nematode troponin C is most similar to troponin C of Drosophila (45% identity) and cardiac troponin C of vertebrates. Expression studies demonstrate that this troponin is expressed in body wall muscle throughout the life of the animal. Later, vulval muscles and anal muscles also express this troponin C isoform. The structural gene for this troponin is pat-10 and mutations in this gene lead to animals that arrest as twofold paralyzed embryos late in development. We have sequenced two of the mutations in pat-10 and both had identical two mutations in the gene; one changes D64 to N and the other changes W153 to a termination site. The missense alteration affects a calcium-binding site and eliminates calcium binding, whereas the second mutation eliminates binding to troponin I. These combined biochemical and in vivo studies of mutant animals demonstrate that this troponin is essential for proper muscle function during development.

    Original languageEnglish (US)
    Pages (from-to)193-202
    Number of pages10
    JournalJournal of Cell Biology
    Issue number1
    StatePublished - Jul 12 1999


    • Caenorhabditis elegans
    • Gene expression
    • Muscle gene
    • Troponin C
    • Troponin I

    ASJC Scopus subject areas

    • Cell Biology


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