Genomic comparison of the closely-related Salmonella enterica serovars Enteritidis, Dublin and Gallinarum

T. David Matthews, Robert Schmieder, Genivaldo G.Z. Silva, Julia Busch, Noriko Cassman, Bas E. Dutilh, Dawn Green, Brian Matlock, Brian Heffernan, Gary J. Olsen, Leigh Farris Hanna, Dieter M. Schifferli, Stanley Maloy, Elizabeth A. Dinsdale, Robert A. Edwards

Research output: Contribution to journalArticle

Abstract

The Salmonella enterica serovars Enteritidis, Dublin, and Gallinarum are closely related but differ in virulence and host range. To identify the genetic elements responsible for these differences and to better understand how these serovars are evolving, we sequenced the genomes of Enteritidis strain LK5 and Dublin strain SARB12 and compared these genomes to the publicly available Enteritidis P125109, Dublin CT 02021853 and Dublin SD3246 genome sequences. We also compared the publicly available Gallinarum genome sequences from biotype Gallinarum 287/91 and Pullorum RKS5078. Using bioinformatic approaches, we identified single nucleotide polymorphisms, insertions, deletions, and differences in prophage and pseudogene content between strains belonging to the same serovar. Through our analysis we also identified several prophage cargo genes and pseudogenes that affect virulence and may contribute to a host-specific, systemic lifestyle. These results strongly argue that the Enteritidis, Dublin and Gallinarum serovars of Salmonella enterica evolve by acquiring new genes through horizontal gene transfer, followed by the formation of pseudogenes. The loss of genes necessary for a gastrointestinal lifestyle ultimately leads to a systemic lifestyle and niche exclusion in the host-specific serovars.

Original languageEnglish (US)
Article numbere0126883
JournalPloS one
Volume10
Issue number6
DOIs
StatePublished - Jun 3 2015

Fingerprint

Salmonella enteritidis
Salmonella
Salmonella Enteritidis
Pseudogenes
pseudogenes
Genes
Genome
lifestyle
Prophages
genomics
Life Style
serotypes
genome
Virulence
virulence
Salmonella Gallinarum
Horizontal Gene Transfer
Salmonella enterica
genes
Host Specificity

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Matthews, T. D., Schmieder, R., Silva, G. G. Z., Busch, J., Cassman, N., Dutilh, B. E., ... Edwards, R. A. (2015). Genomic comparison of the closely-related Salmonella enterica serovars Enteritidis, Dublin and Gallinarum. PloS one, 10(6), [e0126883]. https://doi.org/10.1371/journal.pone.0126883

Genomic comparison of the closely-related Salmonella enterica serovars Enteritidis, Dublin and Gallinarum. / Matthews, T. David; Schmieder, Robert; Silva, Genivaldo G.Z.; Busch, Julia; Cassman, Noriko; Dutilh, Bas E.; Green, Dawn; Matlock, Brian; Heffernan, Brian; Olsen, Gary J.; Hanna, Leigh Farris; Schifferli, Dieter M.; Maloy, Stanley; Dinsdale, Elizabeth A.; Edwards, Robert A.

In: PloS one, Vol. 10, No. 6, e0126883, 03.06.2015.

Research output: Contribution to journalArticle

Matthews, TD, Schmieder, R, Silva, GGZ, Busch, J, Cassman, N, Dutilh, BE, Green, D, Matlock, B, Heffernan, B, Olsen, GJ, Hanna, LF, Schifferli, DM, Maloy, S, Dinsdale, EA & Edwards, RA 2015, 'Genomic comparison of the closely-related Salmonella enterica serovars Enteritidis, Dublin and Gallinarum', PloS one, vol. 10, no. 6, e0126883. https://doi.org/10.1371/journal.pone.0126883
Matthews TD, Schmieder R, Silva GGZ, Busch J, Cassman N, Dutilh BE et al. Genomic comparison of the closely-related Salmonella enterica serovars Enteritidis, Dublin and Gallinarum. PloS one. 2015 Jun 3;10(6). e0126883. https://doi.org/10.1371/journal.pone.0126883
Matthews, T. David ; Schmieder, Robert ; Silva, Genivaldo G.Z. ; Busch, Julia ; Cassman, Noriko ; Dutilh, Bas E. ; Green, Dawn ; Matlock, Brian ; Heffernan, Brian ; Olsen, Gary J. ; Hanna, Leigh Farris ; Schifferli, Dieter M. ; Maloy, Stanley ; Dinsdale, Elizabeth A. ; Edwards, Robert A. / Genomic comparison of the closely-related Salmonella enterica serovars Enteritidis, Dublin and Gallinarum. In: PloS one. 2015 ; Vol. 10, No. 6.
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