Genomic and phylogenetic characterization of novel, recombinant H5N2 avian influenza virus strains isolated from vaccinated chickens with clinical symptoms in China

Huaiying Xu, Fang Meng, Dihai Huang, Xiaodan Sheng, Youling Wang, Wei Zhang, Weishan Chang, Leyi Wang, Zhuoming Qin

Research output: Contribution to journalArticlepeer-review

Abstract

Infection of poultry with diverse lineages of H5N2 avian influenza viruses has been documented for over three decades in different parts of the world, with limited outbreaks caused by this highly pathogenic avian influenza virus. In the present study, three avian H5N2 influenza viruses, A/chicken/Shijiazhuang/1209/2013, A/chicken/Chiping/0321/2014, and A/chicken/Laiwu/0313/2014, were isolated from chickens with clinical symptoms of avian influenza. Complete genomic and phylogenetic analyses demonstrated that all three isolates are novel recombinant viruses with hemagglutinin (HA) and matrix (M) genes derived from H5N1, and remaining genes derived from H9N2-like viruses. The HA cleavage motif in all three strains (PQIEGRRRKR/GL) is characteristic of a highly pathogenic avian influenza virus strain. These results indicate the occurrence of H5N2 recombination and highlight the importance of continued surveillance of the H5N2 subtype virus and reformulation of vaccine strains.

Original languageEnglish (US)
Pages (from-to)889-897
Number of pages9
JournalViruses
Volume7
Issue number3
DOIs
StatePublished - Feb 25 2015
Externally publishedYes

Keywords

  • Avian influenza virus
  • Genomic characterization
  • H5N2
  • Recombination

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

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