Genome-wide expression of low temperature response genes in Rosa hybrida L.

Michele Valquíria dos Reis, Laura Vaughn Rouhana, Ahmed Sadeque, Lucimara Koga, Steven J. Clough, Bernanda Calla, Patrícia Duarte de Oliveira Paiva, Schuyler S. Korban

Research output: Contribution to journalArticle

Abstract

Plants respond to low temperature stress during cold acclimation, a complex process involving changes in physiological and biochemical modifications. The rose serves as a good model to investigate low temperature responses in perennial ornamentals. In this study, a heterologous apple microarray is used to investigate genome-wide expression profiles in Rosa hybrida subjected to low temperature dark treatment. Transcriptome profiles are determined in floral buds at 0h, 2h, and 12h of low temperature treatment (4 °C). It is observed that a total of 134 transcripts are up-regulated and 169 transcripts are down-regulated in response to low temperature. Interestingly, a total of eight up-regulated genes, including those coding for two cytochrome P450 proteins, two ankyrin repeat family proteins, two metal ion binding proteins, and two zinc finger protein-related transcription factors, along with a single down-regulated gene, coding for a dynamin-like protein, are detected. Transcript profiles of 12 genes known to be involved in cold stress response are also validated using qRT-PCR. Furthermore, expression patterns of the AP2/ERF gene family of transcription factors are investigated in both floral buds and leaves. Overall, AP2/ERFs genes are more rapidly induced in leaves than in floral buds. Moreover, differential expression of several AP2/ERF genes are detected earlier in vegetative rather than in reproductive tissues. These findings highlight important roles of various low temperature response genes in mediating cold acclimation, thereby allowing roses to adapt to low temperatures, but without adversely affecting flower bud development and subsequent flowering, while vegetative tissues undergo early adaptation to low temperatures.

Original languageEnglish (US)
Pages (from-to)238-248
Number of pages11
JournalPlant Physiology and Biochemistry
Volume146
DOIs
StatePublished - Jan 2020

Keywords

  • Abiotic stress
  • Cold acclimation
  • Cold temperature response genes
  • Floral buds
  • Rose
  • Transcriptome analysis
  • Vegetative tissues

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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