Genome-wide dynamics of chromatin binding of estrogen receptors α and β: Mutual restriction and competitive site selection

Tze Howe Charn, Edison Tak Bun Liu, Edmund C. Chang, Yew Kok Lee, John A. Katzenellenbogen, Benita S. Katzenellenbogen

Research output: Contribution to journalArticle

Abstract

Estrogen receptors ERα and ERβ, members of the nuclear receptor superfamily, exert profound effects on the gene expression and biological response programs of their target cells. Herein, we explore the dynamic interplay between these two receptors in their selection of chromatin binding sites when present separately or together in MCF-7 breast cancer cells. Treatment of cells (containing ERα only, ERβ only, or ERα and ERβ) with estradiol or ER subtype-selective ligands was followed by chromatin immunoprecipitation analysis with a custom-designed tiling array for ER binding sites across the genome to examine the effects of ligand-occupied and unoccupied ERα and ERβ on chromatin binding. There was substantial overlap in binding sites for these estradiol-liganded nuclear receptors when present alone, but many fewer sites were shared when both ERs were present. Each ER restricted the binding site occupancy of the other, with ERα generally being dominant. Binding sites of both receptors were highly enriched in estrogen response element motifs, but when both ERs were present, ERα displaced ERβ, shifting it into new sites less enriched in estrogen response elements. Binding regions of the two ERs also showed differences in their enrichments for other transcription factor binding motifs. Studies with ER subtype-specific ligands revealed that it was the liganded subtype that principally determined the spectrum of chromatin binding. These findings highlight the dynamic interplay between the two ERs in their selection of chromatin binding sites, with competition, restriction, and site shifting having important implications for the regulation of gene expression by these two nuclear receptors.

Original languageEnglish (US)
Pages (from-to)47-59
Number of pages13
JournalMolecular Endocrinology
Volume24
Issue number1
DOIs
StatePublished - Jan 1 2010

Fingerprint

Estrogen Receptors
Chromatin
Binding Sites
Genome
Cytoplasmic and Nuclear Receptors
Response Elements
Ligands
Estradiol
Estrogens
Chromatin Immunoprecipitation
Gene Expression Regulation
Transcription Factors
Breast Neoplasms
Gene Expression

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

Cite this

Genome-wide dynamics of chromatin binding of estrogen receptors α and β : Mutual restriction and competitive site selection. / Charn, Tze Howe; Liu, Edison Tak Bun; Chang, Edmund C.; Lee, Yew Kok; Katzenellenbogen, John A.; Katzenellenbogen, Benita S.

In: Molecular Endocrinology, Vol. 24, No. 1, 01.01.2010, p. 47-59.

Research output: Contribution to journalArticle

Charn, Tze Howe ; Liu, Edison Tak Bun ; Chang, Edmund C. ; Lee, Yew Kok ; Katzenellenbogen, John A. ; Katzenellenbogen, Benita S. / Genome-wide dynamics of chromatin binding of estrogen receptors α and β : Mutual restriction and competitive site selection. In: Molecular Endocrinology. 2010 ; Vol. 24, No. 1. pp. 47-59.
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