Genome-wide dissection of sex determination genes in the highly invasive whitefly species Bemisia tabaci Q/MED

Y. Liu, W. Xie, L. Guo, X. Yang, J. Yang, S. Wang, Q. Wu, X. Zhou, Y. Zhang

Research output: Contribution to journalArticlepeer-review

Abstract

Sex determination genes are important regulators of reproduction as well as of the development of both behavioural and morphological sex characteristics. The sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is an emerging insect pest worldwide. The recent release of the genome sequence of the highly invasive B. tabaciQ/MED allows us to investigate the mechanisms and genes involved in sex determination. The combined genome and transcriptome-wide analyses identified 26 genes putatively associated with sex determination in B. tabaciQ/MED. The temporal profiles of these genes exhibited a consistent expression pattern at all B. tabaci developmental stages: the highest transcript levels were detected in eggs (21 genes, 80.8%) and the lowest in adults (24 genes, 92.3%). The expression pattern was further validated by quantitative real-time reverse transcription PCR analysis. Alternative splicing analysis found that (1) da and mle have sex-specific isoforms in B. tabaci adults, whereas Imp does not, and (2) exon skipping is a common splicing mechanism involved in B. tabaci sex determination. This research provides a comprehensive list of genes involved in B. tabaci sex determination and provides an opportunity to further understand the mechanisms underlying sex determination in a globally invasive insect pest that reproduces both sexually and asexually.

Original languageEnglish (US)
Pages (from-to)509-519
Number of pages11
JournalInsect Molecular Biology
Volume28
Issue number4
DOIs
StatePublished - Aug 2019
Externally publishedYes

Keywords

  • annotation
  • Bemisia tabaci
  • genome
  • sex determination
  • transcriptome

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Insect Science

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