Genome-wide analysis of phenobarbital-inducible genes in Drosophila melanogaster

W. Sun; V. M. Margam; L. Sun; G. Buczkowski; G. W. Bennett; B. Schemerhorn; W. M. Muir; B. R. Pittendrigh

doi:10.1111/j.1365-2583.2006.00662.x

Genome-wide analysis of phenobarbital-inducible genes in Drosophila melanogaster

W. Sun, V. M. Margam, L. Sun, G. Buczkowski, G. W. Bennett, B. Schemerhorn, W. M. Muir, B. R. Pittendrigh

Research output: Contribution to journal › Article › peer-review

Abstract

An oligoarray analysis was conducted to determine the differential expression of genes due to phenobarbital exposure in Drosophila melanogaster (w1118 strain) third instar larvae. Seventeen genes were observed to be induced with increased expression by a statistical analysis of microarrays approach with a q ≤ 0.05. At q ≤ 0.12, four more genes (Cyp12d1, DmGstd4, and two genes with unknown function) were found to be up-regulated, and 11 genes with unknown function were found to be down-regulated. Fifteen of these genes, Cyp4d14, Cyp6a2, Cyp6a8, Cyp12d1, Cyp6d5, Cyp6w1, CG2065, DmGstd6, DmGstd7, Amy-p/Amy-d, Ugt86Dd, GC5724, Jheh1, Jheh2 and CG11893, were verified using quantitative real time polymerase chain reaction. Some of these genes have been shown to be over-transcribed in metabolically DDT-resistant Drosophila strains.

Original language	English (US)
Pages (from-to)	455-464
Number of pages	10
Journal	Insect Molecular Biology
Volume	15
Issue number	4
DOIs	https://doi.org/10.1111/j.1365-2583.2006.00662.x
State	Published - Aug 2006
Externally published	Yes

Keywords

Carbohydrate metabolism
GST
Oxido-reductase
P450

ASJC Scopus subject areas

Molecular Biology
Genetics
Insect Science

Online availability

10.1111/j.1365-2583.2006.00662.x

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title = "Genome-wide analysis of phenobarbital-inducible genes in Drosophila melanogaster",

abstract = "An oligoarray analysis was conducted to determine the differential expression of genes due to phenobarbital exposure in Drosophila melanogaster (w1118 strain) third instar larvae. Seventeen genes were observed to be induced with increased expression by a statistical analysis of microarrays approach with a q ≤ 0.05. At q ≤ 0.12, four more genes (Cyp12d1, DmGstd4, and two genes with unknown function) were found to be up-regulated, and 11 genes with unknown function were found to be down-regulated. Fifteen of these genes, Cyp4d14, Cyp6a2, Cyp6a8, Cyp12d1, Cyp6d5, Cyp6w1, CG2065, DmGstd6, DmGstd7, Amy-p/Amy-d, Ugt86Dd, GC5724, Jheh1, Jheh2 and CG11893, were verified using quantitative real time polymerase chain reaction. Some of these genes have been shown to be over-transcribed in metabolically DDT-resistant Drosophila strains.",

keywords = "Carbohydrate metabolism, GST, Oxido-reductase, P450",

author = "W. Sun and Margam, {V. M.} and L. Sun and G. Buczkowski and Bennett, {G. W.} and B. Schemerhorn and Muir, {W. M.} and Pittendrigh, {B. R.}",

year = "2006",

month = aug,

doi = "10.1111/j.1365-2583.2006.00662.x",

language = "English (US)",

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AU - Sun, W.

AU - Margam, V. M.

AU - Sun, L.

AU - Buczkowski, G.

AU - Bennett, G. W.

AU - Schemerhorn, B.

AU - Muir, W. M.

AU - Pittendrigh, B. R.

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Y1 - 2006/8

N2 - An oligoarray analysis was conducted to determine the differential expression of genes due to phenobarbital exposure in Drosophila melanogaster (w1118 strain) third instar larvae. Seventeen genes were observed to be induced with increased expression by a statistical analysis of microarrays approach with a q ≤ 0.05. At q ≤ 0.12, four more genes (Cyp12d1, DmGstd4, and two genes with unknown function) were found to be up-regulated, and 11 genes with unknown function were found to be down-regulated. Fifteen of these genes, Cyp4d14, Cyp6a2, Cyp6a8, Cyp12d1, Cyp6d5, Cyp6w1, CG2065, DmGstd6, DmGstd7, Amy-p/Amy-d, Ugt86Dd, GC5724, Jheh1, Jheh2 and CG11893, were verified using quantitative real time polymerase chain reaction. Some of these genes have been shown to be over-transcribed in metabolically DDT-resistant Drosophila strains.

AB - An oligoarray analysis was conducted to determine the differential expression of genes due to phenobarbital exposure in Drosophila melanogaster (w1118 strain) third instar larvae. Seventeen genes were observed to be induced with increased expression by a statistical analysis of microarrays approach with a q ≤ 0.05. At q ≤ 0.12, four more genes (Cyp12d1, DmGstd4, and two genes with unknown function) were found to be up-regulated, and 11 genes with unknown function were found to be down-regulated. Fifteen of these genes, Cyp4d14, Cyp6a2, Cyp6a8, Cyp12d1, Cyp6d5, Cyp6w1, CG2065, DmGstd6, DmGstd7, Amy-p/Amy-d, Ugt86Dd, GC5724, Jheh1, Jheh2 and CG11893, were verified using quantitative real time polymerase chain reaction. Some of these genes have been shown to be over-transcribed in metabolically DDT-resistant Drosophila strains.

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Genome-wide analysis of phenobarbital-inducible genes in Drosophila melanogaster

Abstract

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