Genome-wide analysis of phenobarbital-inducible genes in Drosophila melanogaster

W. Sun, V. M. Margam, L. Sun, G. Buczkowski, G. W. Bennett, B. Schemerhorn, W. M. Muir, B. R. Pittendrigh

Research output: Contribution to journalArticlepeer-review


An oligoarray analysis was conducted to determine the differential expression of genes due to phenobarbital exposure in Drosophila melanogaster (w1118 strain) third instar larvae. Seventeen genes were observed to be induced with increased expression by a statistical analysis of microarrays approach with a q ≤ 0.05. At q ≤ 0.12, four more genes (Cyp12d1, DmGstd4, and two genes with unknown function) were found to be up-regulated, and 11 genes with unknown function were found to be down-regulated. Fifteen of these genes, Cyp4d14, Cyp6a2, Cyp6a8, Cyp12d1, Cyp6d5, Cyp6w1, CG2065, DmGstd6, DmGstd7, Amy-p/Amy-d, Ugt86Dd, GC5724, Jheh1, Jheh2 and CG11893, were verified using quantitative real time polymerase chain reaction. Some of these genes have been shown to be over-transcribed in metabolically DDT-resistant Drosophila strains.

Original languageEnglish (US)
Pages (from-to)455-464
Number of pages10
JournalInsect Molecular Biology
Issue number4
StatePublished - Aug 2006
Externally publishedYes


  • Carbohydrate metabolism
  • GST
  • Oxido-reductase
  • P450

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Insect Science


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