Genome-wide analysis of genes associated with moderate and high DDT resistance in Drosophila melanogaster

Xinghui Qiu, Weilin Sun, Cynthia M. Mcdonnell, Hongmei Li-Byarlay, Laura D. Steele, Jing Wu, Jun Xie, William M. Muir, Barry R. Pittendrigh

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Moderate to high DDT resistance in generally associated with overexpression of multiple genes and therefore has been considered to be polygenic. However, very little information is available about the molecular mechanisms that insect populations employ when evolving increased levels of resistance. The presence of common regulatory motifs among resistance-associated genes may help to explain how and why certain suites of genes are preferentially represented in genomic-scale analyses. Results: A set of commonly differentially expressed genes associated with DDT resistance in the fruit fly was identified on the basis of genome-wide microarray analysis followed by qRT-PCR verification. More genes were observed to be overtranscribed in the highly resistant strain (91-R) than in the moderately resistant strain (Wisconsin) and susceptible strain (Canton-S). Furthermore, possible transcription factor binding sites that occurred in coexpressed resistance-associated genes were discovered by computational motif discovery methods. Conclusion: A glucocorticoid receptor (GR)-like putative transcription factor binding motif (TFBM) was observed to be associated with genes commonly differentially transcribed in both the 91-R and Wisconsin lines of DDT-resistant Drosophila.

Original languageEnglish (US)
Pages (from-to)930-937
Number of pages8
JournalPest Management Science
Volume69
Issue number8
DOIs
StatePublished - Aug 2013
Externally publishedYes

Keywords

  • Dichlorodiphenyltrichloroethane (DDT)
  • Gene transcription
  • Pesticide resistance
  • Transcription factor binding motif

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Insect Science

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