Genome size of Eperythrozoon suis and hybridzation with 16S rRNA gene

J. B. Messick, G. Smith, L. Berent, S. Cooper

Research output: Contribution to journalArticlepeer-review


The genome size of Eperythrozoon suis, an unculturable haemotropic mycoplasma, was estimated using pulsed-field gel electrophoresis (PFGE). Gamma irradiation was used to introduce one (on the average) double-strand break in the E. suis Illinois chromosome. Restriction enzymes that cut infrequently were also used to analyze genome size. The size estimate for the full-length genome was 745 kilobases (kb), whereas the size estimates based on the summation of restriction fragments ranged from 730 to 770 kb. The 16S rRNA gene was located on the 120-kb MluI fragment, 128-kb NruI fragment, 25-kb SacII fragment, and 217-kb SalI fragment by Southern blotting.

Original languageEnglish (US)
Pages (from-to)1082-1086
Number of pages5
JournalCanadian journal of microbiology
Issue number11
StatePublished - 2000


  • 16S rRNA
  • Eperythrozoon suis
  • Genome size
  • Mycoplasma pneumoniae group
  • Pulsed-field gel electrophoresis

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • Biochemistry
  • Biotechnology
  • Immunology
  • Microbiology


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