@article{80e7e60c9a8d4d65bd60396681107a50,
title = "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis",
abstract = "Xylose is a major constituent of plant lignocellulose, and its fermentation is important for the bioconversion of plant biomass to fuels and chemicals. Pichia stipitis is a well-studied, native xylose-fermenting yeast. The mechanism and regulation of xylose metabolism in P. stipitis have been characterized and genes from P. stipitis have been used to engineer xylose metabolism in Saccharomyces cerevisiae. We have sequenced and assembled the complete genome of P. stipitis. The sequence data have revealed unusual aspects of genome organization, numerous genes for bioconversion, a preliminary insight into regulation of central metabolic pathways and several examples of colocalized genes with related functions. The genome sequence provides insight into how P. stipitis regulates its redox balance while very efficiently fermenting xylose under microaerobic conditions.",
author = "Jeffries, {Thomas W.} and Grigoriev, {Igor V.} and Jane Grimwood and Laplaza, {Jos{\'e} M.} and Andrea Aerts and Asaf Salamov and Jeremy Schmutz and Erika Lindquist and Paramvir Dehal and Harris Shapiro and Jin, {Yong Su} and Volkmar Passoth and Richardson, {Paul M.}",
note = "Funding Information: This work was performed under the auspices of the US Department of Energy{\textquoteright}s Office of Science, Biological and Environmental Research Program, and by the University of California, Lawrence Livermore National Laboratory under Contract No. W-7405-Eng-48; Lawrence Berkeley National Laboratory under contract No. DE-AC02-05CH11231; Los Alamos National Laboratory under contract No. W-7405-ENG-36; Stanford University under contract No. DE-FC02-99ER62873, and by the US, Forest Service, Forest Products Laboratory. The authors are grateful to C.P. Kurtzman of the USDA ARS Culture Collection (NRRL) for providing the P. stipitis stock culture, to W. Huang, G. Werner and his group of the JGI for engineering support of annotation, to A. Polyakov and I. Dubchak of the JGI for VISTA analysis, to A. Darling for advice and support in MAUVE analysis, W. R. Kenealy, T. A. Kuster and Mark Davis of the USDA Forest Products Laboratory for carrying out continuous culture studies, providing photomicrographs and analyzing fermentation products, Samuel Pitluck and Kemin Zhou of JGI for assistance with the GenBank submission and to James Cregg, and Lisbeth Olsson and Jennifer Headman Van Vleet for critical readings of early drafts.",
year = "2007",
month = mar,
doi = "10.1038/nbt1290",
language = "English (US)",
volume = "25",
pages = "319--326",
journal = "Nature Biotechnology",
issn = "1087-0156",
publisher = "Nature Publishing Group",
number = "3",
}