Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis

Thomas W. Jeffries, Igor V. Grigoriev, Jane Grimwood, José M. Laplaza, Andrea Aerts, Asaf Salamov, Jeremy Schmutz, Erika Lindquist, Paramvir Dehal, Harris Shapiro, Yong Su Jin, Volkmar Passoth, Paul M. Richardson

Research output: Contribution to journalArticle

Abstract

Xylose is a major constituent of plant lignocellulose, and its fermentation is important for the bioconversion of plant biomass to fuels and chemicals. Pichia stipitis is a well-studied, native xylose-fermenting yeast. The mechanism and regulation of xylose metabolism in P. stipitis have been characterized and genes from P. stipitis have been used to engineer xylose metabolism in Saccharomyces cerevisiae. We have sequenced and assembled the complete genome of P. stipitis. The sequence data have revealed unusual aspects of genome organization, numerous genes for bioconversion, a preliminary insight into regulation of central metabolic pathways and several examples of colocalized genes with related functions. The genome sequence provides insight into how P. stipitis regulates its redox balance while very efficiently fermenting xylose under microaerobic conditions.

Original languageEnglish (US)
Pages (from-to)319-326
Number of pages8
JournalNature Biotechnology
Volume25
Issue number3
DOIs
StatePublished - Mar 1 2007
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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    Jeffries, T. W., Grigoriev, I. V., Grimwood, J., Laplaza, J. M., Aerts, A., Salamov, A., Schmutz, J., Lindquist, E., Dehal, P., Shapiro, H., Jin, Y. S., Passoth, V., & Richardson, P. M. (2007). Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis. Nature Biotechnology, 25(3), 319-326. https://doi.org/10.1038/nbt1290