Genome sequence comparison and superinfection between two related Pseudomonas aeruginosa phages, D3112 and MP22

Yun Jeong Heo, In Young Chung, Kelly B. Choi, Gee Lau, You Hee Cho

Research output: Contribution to journalArticle

Abstract

A temperate transposable bacteriophage (MP22) was isolated from a Korean clinical isolate of Pseudomonas aeruginosa. It has a coliphage λ-like morphology and a double-stranded DNA genome. The complete nucleotide sequence and annotation of the MP22 genome and its characteristics are presented. The MP22 genome is 36 409 bp long with a G + C content of 64.2 mol%. The genome contains 51 proposed ORFs, of which 48 (94%) display synteny and significant nucleotide and protein sequence similarity to the corresponding ORFs of the closely related phage, D3112. Three of the predicted ORFs are unique proteins, whose functions are yet to be revealed. The phage c repressors exhibit striking dissimilarities and, when present as a single gene, did not show cross-immunity. In contrast, although an MP22 lysogen could be productively infected with D3112, MP22 could not grow on a D3112 lysogen, indicating a role of other D3112 genes in superinfection exclusion.

Original languageEnglish (US)
Pages (from-to)2885-2895
Number of pages11
JournalMicrobiology
Volume153
Issue number9
DOIs
StatePublished - Sep 1 2007

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Pseudomonas Phages
Superinfection
Pseudomonas aeruginosa
Genome
Bacteriophages
Open Reading Frames
Coliphages
Synteny
Base Composition
Genes
Immunity
Proteins
DNA

ASJC Scopus subject areas

  • Microbiology

Cite this

Genome sequence comparison and superinfection between two related Pseudomonas aeruginosa phages, D3112 and MP22. / Heo, Yun Jeong; Chung, In Young; Choi, Kelly B.; Lau, Gee; Cho, You Hee.

In: Microbiology, Vol. 153, No. 9, 01.09.2007, p. 2885-2895.

Research output: Contribution to journalArticle

Heo, Yun Jeong ; Chung, In Young ; Choi, Kelly B. ; Lau, Gee ; Cho, You Hee. / Genome sequence comparison and superinfection between two related Pseudomonas aeruginosa phages, D3112 and MP22. In: Microbiology. 2007 ; Vol. 153, No. 9. pp. 2885-2895.
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