Genome-scale phylogeny and comparative genomics of the fungal order Sordariales

Noah Hensen, Lucas Bonometti, Ivar Westerberg, Ioana Onut Brännström, Sonia Guillou, Sandrine Cros-Aarteil, Sara Calhoun, Sajeet Haridas, Alan Kuo, Stephen Mondo, Jasmyn Pangilinan, Robert Riley, Kurt LaButti, Bill Andreopoulos, Anna Lipzen, Cindy Chen, Mi Yan, Chris Daum, Vivian Ng, Alicia ClumAndrei Steindorff, Robin A. Ohm, Francis Martin, Philippe Silar, Donald O. Natvig, Christophe Lalanne, Valérie Gautier, Sandra Lorena Ament-Velásquez, Åsa Kruys, Miriam I. Hutchinson, Amy Jo Powell, Kerrie Barry, Andrew N. Miller, Igor V. Grigoriev, Robert Debuchy, Pierre Gladieux, Markus Hiltunen Thorén, Hanna Johannesson

Research output: Contribution to journalArticlepeer-review

Abstract

The order Sordariales is taxonomically diverse, and harbours many species with different lifestyles and large economic importance. Despite its importance, a robust genome-scale phylogeny, and associated comparative genomic analysis of the order is lacking. In this study, we examined whole-genome data from 99 Sordariales, including 52 newly sequenced genomes, and seven outgroup taxa. We inferred a comprehensive phylogeny that resolved several contentious relationships amongst families in the order, and cleared-up intrafamily relationships within the Podosporaceae. Extensive comparative genomics showed that genomes from the three largest families in the dataset (Chaetomiaceae, Podosporaceae and Sordariaceae) differ greatly in GC content, genome size, gene number, repeat percentage, evolutionary rate, and genome content affected by repeat-induced point mutations (RIP). All genomic traits showed phylogenetic signal, and ancestral state reconstruction revealed that the variation of the properties stems primarily from within-family evolution. Together, the results provide a thorough framework for understanding genome evolution in this important group of fungi.

Original languageEnglish (US)
Article number107938
JournalMolecular Phylogenetics and Evolution
Volume189
DOIs
StatePublished - Dec 2023
Externally publishedYes

Keywords

  • Chaetomiaceae
  • Genome evolution
  • Podosporaceae
  • Sordariaceae
  • Whole-genome phylogeny

ASJC Scopus subject areas

  • Genetics
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology

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