Abstract
We developed a CRISPR-Cas9- and homology-directed-repair-assisted genome-scale engineering method named CHAnGE that can rapidly output tens of thousands of specific genetic variants in yeast. More than 98% of target sequences were efficiently edited with an average frequency of 82%. We validate the single-nucleotide resolution genome-editing capability of this technology by creating a genome-wide gene disruption collection and apply our method to improve tolerance to growth inhibitors.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 505-508 |
| Number of pages | 4 |
| Journal | Nature Biotechnology |
| Volume | 36 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jul 1 2018 |
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
- Molecular Medicine
- Biomedical Engineering
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Cite this
Bao, Z., HamediRad, M., Xue, P., Xiao, H., Tasan, I., Chao, R., Liang, J., & Zhao, H. (2018). Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision. Nature Biotechnology, 36(6), 505-508. https://doi.org/10.1038/nbt.4132