Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision

Zehua Bao, Mohammad HamediRad, Pu Xue, Han Xiao, Ipek Tasan, Ran Chao, Jing Liang, Huimin Zhao

Research output: Contribution to journalArticle

Abstract

We developed a CRISPR-Cas9- and homology-directed-repair-assisted genome-scale engineering method named CHAnGE that can rapidly output tens of thousands of specific genetic variants in yeast. More than 98% of target sequences were efficiently edited with an average frequency of 82%. We validate the single-nucleotide resolution genome-editing capability of this technology by creating a genome-wide gene disruption collection and apply our method to improve tolerance to growth inhibitors.

Original languageEnglish (US)
Pages (from-to)505-508
Number of pages4
JournalNature Biotechnology
Volume36
Issue number6
DOIs
StatePublished - Jul 1 2018

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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  • Cite this

    Bao, Z., HamediRad, M., Xue, P., Xiao, H., Tasan, I., Chao, R., Liang, J., & Zhao, H. (2018). Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision. Nature Biotechnology, 36(6), 505-508. https://doi.org/10.1038/nbt.4132