@article{8d54bd57ab5f456b915174b4ebd6700c,
title = "Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision",
abstract = "We developed a CRISPR-Cas9- and homology-directed-repair-assisted genome-scale engineering method named CHAnGE that can rapidly output tens of thousands of specific genetic variants in yeast. More than 98% of target sequences were efficiently edited with an average frequency of 82%. We validate the single-nucleotide resolution genome-editing capability of this technology by creating a genome-wide gene disruption collection and apply our method to improve tolerance to growth inhibitors.",
author = "Zehua Bao and Mohammad HamediRad and Pu Xue and Han Xiao and Ipek Tasan and Ran Chao and Jing Liang and Huimin Zhao",
note = "Funding Information: 505 508 10.1038/nbt.4132 EN Zehua Bao Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. http://orcid.org/0000-0002-5519-4864 Mohammad HamediRad Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. http://orcid.org/0000-0002-8604-1713 Pu Xue Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. http://orcid.org/0000-0001-6023-1298 Han Xiao Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. Ipek Tasan Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. Ran Chao Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. Jing Liang Metabolic Engineering Research Laboratory, Science and Engineering Institutes, Agency for Science, Technology and Research, Singapore, Singapore. Huimin Zhao Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. Metabolic Engineering Research Laboratory, Science and Engineering Institutes, Agency for Science, Technology and Research, Singapore, Singapore. Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. http://orcid.org/0000-0002-9069-6739 nbt.4132 10.1038/nbt.4132 2017 05 01 2018 03 02 2018 May 07 We developed a CRISPR–Cas9- and homology-directed-repair-assisted genome-scale engineering method named CHAnGE that can rapidly output tens of thousands of specific genetic variants in yeast. More than 98% of target sequences were efficiently edited with an average frequency of 82%. We validate the single-nucleotide resolution genome-editing capability of this technology by creating a genome-wide gene disruption collection and apply our method to improve tolerance to growth inhibitors. Publisher Copyright: {\textcopyright} 2018 Nature America, Inc. part of Springer Nature. All rights reserved.",
year = "2018",
month = jul,
day = "1",
doi = "10.1038/nbt.4132",
language = "English (US)",
volume = "36",
pages = "505--508",
journal = "Nature Biotechnology",
issn = "1087-0156",
publisher = "Nature Publishing Group",
number = "6",
}