@inbook{c91ddf6982834ef0acdac75e369d8c83,
title = "Genome engineering with custom recombinases",
abstract = "Site-specific recombinases are valuable tools for myriad basic research and genome engineering applications. In particular, hybrid recombinases consisting of catalytic domains from the resolvase/invertase family of serine recombinases fused to Cys2-His2 zinc-finger or TAL effector DNA-binding domains are capable of introducing targeted modifications into mammalian cells. Due to their inherent modularity, new recombinases with distinct targeting specificities can readily be generated and utilized in a {"}plug-and-play{"} manner. In this protocol, we provide detailed, step-by-step instructions for generating new hybrid recombinases with user-defined specificity, as well as methods for achieving site-specific integration into targeted genomic loci using these systems.",
keywords = "Genome engineering, Protein engineering, Recombinase, TAL effector, Zinc-finger",
author = "Thomas Gaj and Barbas, {Carlos F.}",
note = "Publisher Copyright: {\textcopyright} 2014 Elsevier Inc.",
year = "2014",
doi = "10.1016/B978-0-12-801185-0.00004-0",
language = "English (US)",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
number = "C",
pages = "79--91",
booktitle = "Methods in Enzymology",
address = "United States",
edition = "C",
}