Genome Editing and CRISPR Technology

Colin K.W. Lim, Thomas Gaj

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Genome editing has revolutionized the life sciences by providing scientists with an efficient means for introducing targeted modifications into DNA. Driving these advances are programmable nucleases, which stimulate gene editing by inducing targeted DNA double-strand breaks (DSBs) that in turn activate cellular DNA repair pathways that can mediate the introduction of specific edits. For most genome engineers, technologies derived from naturally occurring CRISPR systems have become the platform of choice as CRISPR is highly versatile and efficient. In this article, we introduce CRISPR technology, describe how it is used to edit DNA and discuss its most common applications.

Original languageEnglish (US)
Title of host publicationEncyclopedia of Cell Biology
Subtitle of host publicationVolume 1-6, Second Edition
PublisherElsevier
Pages650-656
Number of pages7
Volume1
ISBN (Electronic)9780128216248
DOIs
StatePublished - Jan 1 2022
Externally publishedYes

Keywords

  • Base editing
  • CRISPR
  • Cas12
  • Cas13
  • Cas9
  • DNA repair
  • Double-Strand break
  • Epigenome editing
  • Gene regulation
  • Genome editing
  • Prime editing

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

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