TY - JOUR
T1 - Genetic technologies for Archaea
AU - Rother, Michael
AU - Metcalf, William W.
N1 - Portions of the work described here were supported by grants to WWM from The National Science Foundation (MCB0212466) and the Department of Energy (DE-FG02-02ER15296). MR was supported through a fellowship from the Deutsche Forschungsgemeinschaft (RO 2445/1-1).
PY - 2005/12
Y1 - 2005/12
N2 - Members of the third domain of life, the Archaea, possess structural, physiological, biochemical and genetic features distinct from Bacteria and Eukarya and, therefore, have drawn considerable scientific interest. Physiological, biochemical and molecular analyses have revealed many novel biological processes in these important prokaryotes. However, assessment of the function of genes in vivo through genetic analysis has lagged behind because suitable systems for the creation of mutants in most Archaea were established only in the past decade. Among the Archaea, sufficiently sophisticated genetic systems now exist for some thermophilic sulfur-metabolizing Archaea, halophilic Archaea and methanogenic Archaea. Recently, there have been developments in genetic analysis of thermophilic and methanogenic Archaea and in the use of genetics to study the physiology, metabolism and regulatory mechanisms that direct gene expression in response to changes of environmental conditions in these important microorganisms.
AB - Members of the third domain of life, the Archaea, possess structural, physiological, biochemical and genetic features distinct from Bacteria and Eukarya and, therefore, have drawn considerable scientific interest. Physiological, biochemical and molecular analyses have revealed many novel biological processes in these important prokaryotes. However, assessment of the function of genes in vivo through genetic analysis has lagged behind because suitable systems for the creation of mutants in most Archaea were established only in the past decade. Among the Archaea, sufficiently sophisticated genetic systems now exist for some thermophilic sulfur-metabolizing Archaea, halophilic Archaea and methanogenic Archaea. Recently, there have been developments in genetic analysis of thermophilic and methanogenic Archaea and in the use of genetics to study the physiology, metabolism and regulatory mechanisms that direct gene expression in response to changes of environmental conditions in these important microorganisms.
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U2 - 10.1016/j.mib.2005.10.010
DO - 10.1016/j.mib.2005.10.010
M3 - Review article
C2 - 16257573
AN - SCOPUS:27844514673
SN - 1369-5274
VL - 8
SP - 745
EP - 751
JO - Current Opinion in Microbiology
JF - Current Opinion in Microbiology
IS - 6
ER -