Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9

Adam Sateriale; Mattie Pawlowic; Sumiti Vinayak; Carrie Brooks; Boris Striepen

doi:10.1007/978-1-4939-9748-0_13

Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9

Adam Sateriale, Mattie Pawlowic, Sumiti Vinayak, Carrie Brooks, Boris Striepen

Pathobiology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Cryptosporidium parvum can be reliably genetically manipulated using CRISPR/Cas9-driven homologous repair coupled to in vivo propagation within immunodeficient mice. Recent modifications have simplified the initial protocol significantly. This chapter will guide through procedures for excystation, transfection, infection, collection, and purification of transgenic Cryptosporidium parvum.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	219-228
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-4939-9748-0_13
State	Published - 2020

Publication series

Name	Methods in Molecular Biology
Volume	2052
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

CRISPR
Cas9
Genetics
Transfection
Transgenics

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-9748-0_13

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Cite this

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