Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9

Adam Sateriale, Mattie Pawlowic, Sumiti Vinayak, Carrie Brooks, Boris Striepen

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Cryptosporidium parvum can be reliably genetically manipulated using CRISPR/Cas9-driven homologous repair coupled to in vivo propagation within immunodeficient mice. Recent modifications have simplified the initial protocol significantly. This chapter will guide through procedures for excystation, transfection, infection, collection, and purification of transgenic Cryptosporidium parvum.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages219-228
Number of pages10
DOIs
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2052
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Fingerprint

Clustered Regularly Interspaced Short Palindromic Repeats
Cryptosporidium parvum
Transfection
Infection

Keywords

  • CRISPR
  • Cas9
  • Genetics
  • Transfection
  • Transgenics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Sateriale, A., Pawlowic, M., Vinayak, S., Brooks, C., & Striepen, B. (2020). Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9. In Methods in Molecular Biology (pp. 219-228). (Methods in Molecular Biology; Vol. 2052). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9748-0_13

Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9. / Sateriale, Adam; Pawlowic, Mattie; Vinayak, Sumiti; Brooks, Carrie; Striepen, Boris.

Methods in Molecular Biology. Humana Press Inc., 2020. p. 219-228 (Methods in Molecular Biology; Vol. 2052).

Research output: Chapter in Book/Report/Conference proceedingChapter

Sateriale, A, Pawlowic, M, Vinayak, S, Brooks, C & Striepen, B 2020, Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 2052, Humana Press Inc., pp. 219-228. https://doi.org/10.1007/978-1-4939-9748-0_13
Sateriale A, Pawlowic M, Vinayak S, Brooks C, Striepen B. Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9. In Methods in Molecular Biology. Humana Press Inc. 2020. p. 219-228. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9748-0_13
Sateriale, Adam ; Pawlowic, Mattie ; Vinayak, Sumiti ; Brooks, Carrie ; Striepen, Boris. / Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9. Methods in Molecular Biology. Humana Press Inc., 2020. pp. 219-228 (Methods in Molecular Biology).
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