Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9

Adam Sateriale, Mattie Pawlowic, Sumiti Vinayak, Carrie Brooks, Boris Striepen

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Cryptosporidium parvum can be reliably genetically manipulated using CRISPR/Cas9-driven homologous repair coupled to in vivo propagation within immunodeficient mice. Recent modifications have simplified the initial protocol significantly. This chapter will guide through procedures for excystation, transfection, infection, collection, and purification of transgenic Cryptosporidium parvum.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages219-228
Number of pages10
DOIs
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2052
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Fingerprint

Clustered Regularly Interspaced Short Palindromic Repeats
Cryptosporidium parvum
Transfection
Infection

Keywords

  • CRISPR
  • Cas9
  • Genetics
  • Transfection
  • Transgenics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Sateriale, A., Pawlowic, M., Vinayak, S., Brooks, C., & Striepen, B. (2020). Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9. In Methods in Molecular Biology (pp. 219-228). (Methods in Molecular Biology; Vol. 2052). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9748-0_13

Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9. / Sateriale, Adam; Pawlowic, Mattie; Vinayak, Sumiti; Brooks, Carrie; Striepen, Boris.

Methods in Molecular Biology. Humana Press Inc., 2020. p. 219-228 (Methods in Molecular Biology; Vol. 2052).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Sateriale, A, Pawlowic, M, Vinayak, S, Brooks, C & Striepen, B 2020, Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 2052, Humana Press Inc., pp. 219-228. https://doi.org/10.1007/978-1-4939-9748-0_13
Sateriale A, Pawlowic M, Vinayak S, Brooks C, Striepen B. Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9. In Methods in Molecular Biology. Humana Press Inc. 2020. p. 219-228. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9748-0_13
Sateriale, Adam ; Pawlowic, Mattie ; Vinayak, Sumiti ; Brooks, Carrie ; Striepen, Boris. / Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9. Methods in Molecular Biology. Humana Press Inc., 2020. pp. 219-228 (Methods in Molecular Biology).
@inbook{9339ed18395a45d39fe90f02a3962966,
title = "Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9",
abstract = "Cryptosporidium parvum can be reliably genetically manipulated using CRISPR/Cas9-driven homologous repair coupled to in vivo propagation within immunodeficient mice. Recent modifications have simplified the initial protocol significantly. This chapter will guide through procedures for excystation, transfection, infection, collection, and purification of transgenic Cryptosporidium parvum.",
keywords = "CRISPR, Cas9, Genetics, Transfection, Transgenics",
author = "Adam Sateriale and Mattie Pawlowic and Sumiti Vinayak and Carrie Brooks and Boris Striepen",
year = "2020",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-9748-0_13",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "219--228",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9

AU - Sateriale, Adam

AU - Pawlowic, Mattie

AU - Vinayak, Sumiti

AU - Brooks, Carrie

AU - Striepen, Boris

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Cryptosporidium parvum can be reliably genetically manipulated using CRISPR/Cas9-driven homologous repair coupled to in vivo propagation within immunodeficient mice. Recent modifications have simplified the initial protocol significantly. This chapter will guide through procedures for excystation, transfection, infection, collection, and purification of transgenic Cryptosporidium parvum.

AB - Cryptosporidium parvum can be reliably genetically manipulated using CRISPR/Cas9-driven homologous repair coupled to in vivo propagation within immunodeficient mice. Recent modifications have simplified the initial protocol significantly. This chapter will guide through procedures for excystation, transfection, infection, collection, and purification of transgenic Cryptosporidium parvum.

KW - CRISPR

KW - Cas9

KW - Genetics

KW - Transfection

KW - Transgenics

UR - http://www.scopus.com/inward/record.url?scp=85071634091&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85071634091&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-9748-0_13

DO - 10.1007/978-1-4939-9748-0_13

M3 - Chapter

C2 - 31452165

AN - SCOPUS:85071634091

T3 - Methods in Molecular Biology

SP - 219

EP - 228

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Access to Document