@inbook{9339ed18395a45d39fe90f02a3962966,
title = "Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9",
abstract = "Cryptosporidium parvum can be reliably genetically manipulated using CRISPR/Cas9-driven homologous repair coupled to in vivo propagation within immunodeficient mice. Recent modifications have simplified the initial protocol significantly. This chapter will guide through procedures for excystation, transfection, infection, collection, and purification of transgenic Cryptosporidium parvum.",
keywords = "CRISPR, Cas9, Genetics, Transfection, Transgenics",
author = "Adam Sateriale and Mattie Pawlowic and Sumiti Vinayak and Carrie Brooks and Boris Striepen",
note = "Publisher Copyright: {\textcopyright} 2020, Springer Science+Business Media, LLC, part of Springer Nature. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.",
year = "2020",
doi = "10.1007/978-1-4939-9748-0_13",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "219--228",
booktitle = "Methods in Molecular Biology",
}