Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9

Adam Sateriale; Mattie Pawlowic; Sumiti Vinayak; Carrie Brooks; Boris Striepen

doi:10.1007/978-1-4939-9748-0_13

Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9

Pathobiology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Cryptosporidium parvum can be reliably genetically manipulated using CRISPR/Cas9-driven homologous repair coupled to in vivo propagation within immunodeficient mice. Recent modifications have simplified the initial protocol significantly. This chapter will guide through procedures for excystation, transfection, infection, collection, and purification of transgenic Cryptosporidium parvum.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	219-228
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-4939-9748-0_13
State	Published - Jan 1 2020

Publication series

Name	Methods in Molecular Biology
Volume	2052
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

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Keywords

CRISPR
Cas9
Genetics
Transfection
Transgenics

ASJC Scopus subject areas

Molecular Biology
Genetics

Cite this

Sateriale, A., Pawlowic, M., Vinayak, S., Brooks, C., & Striepen, B. (2020). Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9. In Methods in Molecular Biology (pp. 219-228). (Methods in Molecular Biology; Vol. 2052). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9748-0_13

TY - CHAP

T1 - Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9

AU - Sateriale, Adam

AU - Pawlowic, Mattie

AU - Vinayak, Sumiti

AU - Brooks, Carrie

AU - Striepen, Boris

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Cryptosporidium parvum can be reliably genetically manipulated using CRISPR/Cas9-driven homologous repair coupled to in vivo propagation within immunodeficient mice. Recent modifications have simplified the initial protocol significantly. This chapter will guide through procedures for excystation, transfection, infection, collection, and purification of transgenic Cryptosporidium parvum.

AB - Cryptosporidium parvum can be reliably genetically manipulated using CRISPR/Cas9-driven homologous repair coupled to in vivo propagation within immunodeficient mice. Recent modifications have simplified the initial protocol significantly. This chapter will guide through procedures for excystation, transfection, infection, collection, and purification of transgenic Cryptosporidium parvum.

KW - CRISPR

KW - Cas9

KW - Genetics

KW - Transfection

KW - Transgenics

UR - http://www.scopus.com/inward/record.url?scp=85071634091&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85071634091&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-9748-0_13

DO - 10.1007/978-1-4939-9748-0_13

M3 - Chapter

C2 - 31452165

AN - SCOPUS:85071634091

T3 - Methods in Molecular Biology

SP - 219

EP - 228

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Access to Document

10.1007/978-1-4939-9748-0_13