@inbook{ae40618f868a4703a748e57c9a27f419,
title = "Genetic Engineering of Therapeutic Phages Using Type III CRISPR-Cas Systems",
abstract = "The functional characterization of “hypothetical” phage genes is a major bottleneck in basic and applied phage research. To compound this issue, the most suitable phages for therapeutic applications—the strictly lytic variety—are largely recalcitrant to classical genetic techniques due to low recombination rates and lack of selectable markers. Here we describe methods for fast and effective phage engineering that rely upon a Type III-A CRISPR-Cas system. In these methods, the CRISPR-Cas system is used as a powerful counterselection tool to isolate rare phage recombinants.",
keywords = "Bacteriophage engineering, CRISPR-Cas10, Phage genome editing, Staphylococci, Type III-A CRISPR-Cas",
author = "Hill, {Courtney M.} and Asma Hatoum-Aslan",
note = "A. H.-A. holds an Investigators in the Pathogenesis of Infectious Disease Award from the Burroughs Wellcome Fund. She is also supported by an NSF/MCB CAREER award [2054755] and NIH/NIAID [R21AI156636-01].",
year = "2024",
doi = "10.1007/978-1-0716-3523-0_18",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "279--299",
booktitle = "Methods in Molecular Biology",
}