Genetic Engineering of Surface Attachment Sites Yields Oriented Protein Monolayers

Patrick S. Stayton, Jill M. Olinger, Min Jiang, Paul W. Bohn, Stephen G. Sligar

Research output: Contribution to journalArticlepeer-review


There has been considerable interest in the use of self-assembly and Langmuir-Blodgett techniques to generate ordered macromolecular monolayers. We describe a general method of using genetic engineering to produce a unique chemical group on the surface of a protein at a predefined site which can be used to orient the macromolecule in a self-assembled film. Using the heme protein cytochrome b5, produced from a totally synthetic gene, allows direct determination of orientation through measurement of absorbance linear dichroism.

Original languageEnglish (US)
Pages (from-to)9298-9299
Number of pages2
JournalJournal of the American Chemical Society
Issue number24
StatePublished - Nov 1 1992

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry
  • Biochemistry
  • Colloid and Surface Chemistry


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