Genetic engineering of membrane lipid

D. F. Silbert, John E Cronan, I. R. Beacham, M. E. Harder

Research output: Contribution to journalArticle

Abstract

Methods for isolating mutant strains of E. coli defective in fatty acid biosynthesis (fab) or in phospholipid synthesis (pls) were developed and some of the mutants characterized with respect to their biochemical defects and their location on the E. coli chromosome. FabA and fabB mutants were employed extensively to define the requirement for cis unsaturated fatty acids in E. coli. Cis unsaturated fatty acids were replaced by certain branched chain fatty acids and at higher growth temperatures in part by intermediate chain length saturated fatty acids without serious loss in membrane function. These findings support the view that the physical properties of the acyl groups are of paramount importance. Utilizing an unsaturated fatty acid auxotroph and various exogenously supplied fatty acid analogues to modify membrane phospholipid structure, UDPgalactose lipopolysaccharide galactosyltransferase is found to be most active under conditions which are known to favor mobility of membrane fatty acyl groups. The differences in activity with the different membrane preparations can be attributed in some instances to differences in the temperature of the thermotropic lipid phase transitions, but reflect in other cases differences in the physical environment of the membrane which persist above the phase transitions.

Original languageEnglish (US)
Pages (from-to)1725-1732
Number of pages8
JournalFederation Proceedings
Volume33
Issue number6
StatePublished - Jan 1 1974
Externally publishedYes

Fingerprint

Genetic Engineering
Membrane Lipids
Fatty Acids
Unsaturated Fatty Acids
Membranes
Phase Transition
Escherichia coli
Phospholipids
Temperature
Chromosomes
Lipids
Growth

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Silbert, D. F., Cronan, J. E., Beacham, I. R., & Harder, M. E. (1974). Genetic engineering of membrane lipid. Federation Proceedings, 33(6), 1725-1732.

Genetic engineering of membrane lipid. / Silbert, D. F.; Cronan, John E; Beacham, I. R.; Harder, M. E.

In: Federation Proceedings, Vol. 33, No. 6, 01.01.1974, p. 1725-1732.

Research output: Contribution to journalArticle

Silbert, DF, Cronan, JE, Beacham, IR & Harder, ME 1974, 'Genetic engineering of membrane lipid', Federation Proceedings, vol. 33, no. 6, pp. 1725-1732.
Silbert DF, Cronan JE, Beacham IR, Harder ME. Genetic engineering of membrane lipid. Federation Proceedings. 1974 Jan 1;33(6):1725-1732.
Silbert, D. F. ; Cronan, John E ; Beacham, I. R. ; Harder, M. E. / Genetic engineering of membrane lipid. In: Federation Proceedings. 1974 ; Vol. 33, No. 6. pp. 1725-1732.
@article{9126adca7dc84504a12ff7e4f4dd66f1,
title = "Genetic engineering of membrane lipid",
abstract = "Methods for isolating mutant strains of E. coli defective in fatty acid biosynthesis (fab) or in phospholipid synthesis (pls) were developed and some of the mutants characterized with respect to their biochemical defects and their location on the E. coli chromosome. FabA and fabB mutants were employed extensively to define the requirement for cis unsaturated fatty acids in E. coli. Cis unsaturated fatty acids were replaced by certain branched chain fatty acids and at higher growth temperatures in part by intermediate chain length saturated fatty acids without serious loss in membrane function. These findings support the view that the physical properties of the acyl groups are of paramount importance. Utilizing an unsaturated fatty acid auxotroph and various exogenously supplied fatty acid analogues to modify membrane phospholipid structure, UDPgalactose lipopolysaccharide galactosyltransferase is found to be most active under conditions which are known to favor mobility of membrane fatty acyl groups. The differences in activity with the different membrane preparations can be attributed in some instances to differences in the temperature of the thermotropic lipid phase transitions, but reflect in other cases differences in the physical environment of the membrane which persist above the phase transitions.",
author = "Silbert, {D. F.} and Cronan, {John E} and Beacham, {I. R.} and Harder, {M. E.}",
year = "1974",
month = "1",
day = "1",
language = "English (US)",
volume = "33",
pages = "1725--1732",
journal = "Federation Proceedings",
issn = "0014-9446",
number = "6",

}

TY - JOUR

T1 - Genetic engineering of membrane lipid

AU - Silbert, D. F.

AU - Cronan, John E

AU - Beacham, I. R.

AU - Harder, M. E.

PY - 1974/1/1

Y1 - 1974/1/1

N2 - Methods for isolating mutant strains of E. coli defective in fatty acid biosynthesis (fab) or in phospholipid synthesis (pls) were developed and some of the mutants characterized with respect to their biochemical defects and their location on the E. coli chromosome. FabA and fabB mutants were employed extensively to define the requirement for cis unsaturated fatty acids in E. coli. Cis unsaturated fatty acids were replaced by certain branched chain fatty acids and at higher growth temperatures in part by intermediate chain length saturated fatty acids without serious loss in membrane function. These findings support the view that the physical properties of the acyl groups are of paramount importance. Utilizing an unsaturated fatty acid auxotroph and various exogenously supplied fatty acid analogues to modify membrane phospholipid structure, UDPgalactose lipopolysaccharide galactosyltransferase is found to be most active under conditions which are known to favor mobility of membrane fatty acyl groups. The differences in activity with the different membrane preparations can be attributed in some instances to differences in the temperature of the thermotropic lipid phase transitions, but reflect in other cases differences in the physical environment of the membrane which persist above the phase transitions.

AB - Methods for isolating mutant strains of E. coli defective in fatty acid biosynthesis (fab) or in phospholipid synthesis (pls) were developed and some of the mutants characterized with respect to their biochemical defects and their location on the E. coli chromosome. FabA and fabB mutants were employed extensively to define the requirement for cis unsaturated fatty acids in E. coli. Cis unsaturated fatty acids were replaced by certain branched chain fatty acids and at higher growth temperatures in part by intermediate chain length saturated fatty acids without serious loss in membrane function. These findings support the view that the physical properties of the acyl groups are of paramount importance. Utilizing an unsaturated fatty acid auxotroph and various exogenously supplied fatty acid analogues to modify membrane phospholipid structure, UDPgalactose lipopolysaccharide galactosyltransferase is found to be most active under conditions which are known to favor mobility of membrane fatty acyl groups. The differences in activity with the different membrane preparations can be attributed in some instances to differences in the temperature of the thermotropic lipid phase transitions, but reflect in other cases differences in the physical environment of the membrane which persist above the phase transitions.

UR - http://www.scopus.com/inward/record.url?scp=0016146711&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016146711&partnerID=8YFLogxK

M3 - Article

C2 - 4597230

AN - SCOPUS:0016146711

VL - 33

SP - 1725

EP - 1732

JO - Federation Proceedings

JF - Federation Proceedings

SN - 0014-9446

IS - 6

ER -