Genetic engineering of insects with mariner transposons

David J. Lampe, Kimberly K.O. Walden, John M. Sherwood, Hugh M. Robertson

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The mariner family of transposons holds considerabie promise for development as genetis tools in insects. Exploitation of this family of transposons generally follcws the model of the P element in Drosophila melangnster however. tere are several features of themariner family thatmake its lnembers particuiarly appropriate as genetic tools for insects. First. they are extremely widespread and diverse in anima: genomes. Second, they persist primarily by repeated horizontal transfers into new host genomes. Third: their transposases are capable of lirnceioning autonomously of host proteins. Fourth. they are capable of fixctioning in diverse host environments. Fifth, their functionig in bacteria such as Escherichia coli allows manipulation of their transposase and inverted terminal repeats to generate improved versions. Sixth, mariner from different subfamilies, and perhaps divergent lineages within subfamilies, do not interact. This chapter reviews some consequences of their prospects as genetic tools for insects.

Original languageEnglish (US)
Title of host publicationInsect Transgenesis
Subtitle of host publicationMethods and Applications
PublisherCRC Press
Pages237-248
Number of pages12
ISBN (Electronic)9781420039399
ISBN (Print)0849320283, 9780849320286
DOIs
StatePublished - Jan 1 2000

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Genetic engineering
Genetic Engineering
genetic engineering
transposons
Transposases
Insects
insects
Genes
Genome
terminal repeat sequences
genome
Terminal Repeat Sequences
Escherichia coli
Drosophila
Bacteria
bacteria
Proteins
proteins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Lampe, D. J., Walden, K. K. O., Sherwood, J. M., & Robertson, H. M. (2000). Genetic engineering of insects with mariner transposons. In Insect Transgenesis: Methods and Applications (pp. 237-248). CRC Press. https://doi.org/10.1201/9781420039399

Genetic engineering of insects with mariner transposons. / Lampe, David J.; Walden, Kimberly K.O.; Sherwood, John M.; Robertson, Hugh M.

Insect Transgenesis: Methods and Applications. CRC Press, 2000. p. 237-248.

Research output: Chapter in Book/Report/Conference proceedingChapter

Lampe, DJ, Walden, KKO, Sherwood, JM & Robertson, HM 2000, Genetic engineering of insects with mariner transposons. in Insect Transgenesis: Methods and Applications. CRC Press, pp. 237-248. https://doi.org/10.1201/9781420039399
Lampe DJ, Walden KKO, Sherwood JM, Robertson HM. Genetic engineering of insects with mariner transposons. In Insect Transgenesis: Methods and Applications. CRC Press. 2000. p. 237-248 https://doi.org/10.1201/9781420039399
Lampe, David J. ; Walden, Kimberly K.O. ; Sherwood, John M. ; Robertson, Hugh M. / Genetic engineering of insects with mariner transposons. Insect Transgenesis: Methods and Applications. CRC Press, 2000. pp. 237-248
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