TY - JOUR
T1 - Genetic Diversity and Structure of Physaria on the Kaibab Plateau
T2 - Implications for Conservation
AU - Chong, Jer Pin
AU - Minnaert-Grote, Jamie
AU - Zaya, David N.
AU - Ashley, Mary V.
AU - Coons, Janice
AU - Ramp Neale, Jennifer M.
AU - Molano-Flores, Brenda
N1 - Funding: This work was supported by U.S. Forest Service, FS Agreement No. 16-CR-11030703-013; Arizona Department of Agriculture U.S. Fish and Wildlife Service Cooperative Endangered Species Conservation Fund (Section\u00A06 of the Endangered Species Act), Segment 18 2014-2016-11 F14APOO869, University of Illinois University Scholar Funds. We thank the Arizona Department of Agriculture for funding this project through the U.S. Fish and Wildlife Service Cooperative Endangered Species Conservation Fund (Section\u00A06 of the Endangered Species Act) Grant Program: Grant No. Segment 18 2014-2016-11 F14APOO869 and the U.S. Forest Service provided additional funding to complete the project (FS Agreement No. 16-CR-11030703-013; Cooperator Agreement No. 2016-06506-00-00). Funding was also provided by the University of Illinois University Scholar Funds (to MVA). We thank Robert Massatti, Shannon Lencioni, Morgan Andrews, Mary Ann Feist, Samantha Primer, Jenna Annis, and Jean Mengelkoch for assisting in field sampling. We also thank Eric Janssen for constructing the sampling map. Finally, we thank Kevin Feldheim and the Pritzker DNA Lab at the Field Museum for the use of their facilities and expertise.
This work was supported by U.S. Forest Service, FS Agreement No. 16\u2010CR\u201011030703\u2010013; Arizona Department of Agriculture U.S. Fish and Wildlife Service Cooperative Endangered Species Conservation Fund (Section 6 of the Endangered Species Act), Segment 18 2014\u20102016\u201011 F14APOO869, University of Illinois University Scholar Funds. Funding:
We thank the Arizona Department of Agriculture for funding this project through the U.S. Fish and Wildlife Service Cooperative Endangered Species Conservation Fund (Section 6 of the Endangered Species Act) Grant Program: Grant No. Segment 18 2014\u20102016\u201011 F14APOO869 and the U.S. Forest Service provided additional funding to complete the project (FS Agreement No. 16\u2010CR\u201011030703\u2010013; Cooperator Agreement No. 2016\u201006506\u201000\u201000). Funding was also provided by the University of Illinois University Scholar Funds (to MVA). We thank Robert Massatti, Shannon Lencioni, Morgan Andrews, Mary Ann Feist, Samantha Primer, Jenna Annis, and Jean Mengelkoch for assisting in field sampling. We also thank Eric Janssen for constructing the sampling map. Finally, we thank Kevin Feldheim and the Pritzker DNA Lab at the Field Museum for the use of their facilities and expertise.
PY - 2024/11
Y1 - 2024/11
N2 - Assessing patterns of genetic diversity within and among closely related congeners is important for evaluating the conservation status of rare plant taxa. We used nuclear microsatellite genotyping to examine the genetic relatedness among three Physaria taxa on the Kaibab Plateau in northern Arizona. Over 100 species of Physaria are recognized, and are widely distributed in Western North America. On the Kaibab Plateau several taxa occur, including Physaria arizonica and two subspecies of P. kingii, P. kingii subsp. latifolia and P. kingii subsp. kaibabensis. The latter subspecies, the Kaibab bladderpod, is rare and endemic to the Kaibab Plateau and is a potential candidate to be listed under the Endangered Species Act. Morphological characters, primarily flower color, have been used to distinguish P. kingii subsp. kaibabensis from other subspecies. Here we aim to assess its genetic diversity and differentiation as compared to congeners on and around the Kaibab Plateau. We genotyped DNA obtained from leaf samples from 463 individuals collected from 26 sites representing the three putative taxa (12 P. kingii subsp. kaibabensis, 8 P. kingii subsp. latifolia, and 6 P. arizonica). Our results showed that all samples initially identified as P. kingii subsp. latifolia and P. kingii subsp. kaibabensis on the Kaibab Plateau form a single genetic cluster that is well-differentiated from P. kingii subsp. latifolia sampled from sites off the plateau or P. arizonica on or off the plateau. For P. kingii on the plateau, our findings do not support the previous subspecies designations based on morphological characters. While additional studies of P. kingii will further resolve taxonomic uncertainties within this species, our findings indicate that the Kaibab Plateau population is genetically diverse and genetically distinct, and federal protection is justified in light of the threats faced on the Kaibab Plateau and its limited range.
AB - Assessing patterns of genetic diversity within and among closely related congeners is important for evaluating the conservation status of rare plant taxa. We used nuclear microsatellite genotyping to examine the genetic relatedness among three Physaria taxa on the Kaibab Plateau in northern Arizona. Over 100 species of Physaria are recognized, and are widely distributed in Western North America. On the Kaibab Plateau several taxa occur, including Physaria arizonica and two subspecies of P. kingii, P. kingii subsp. latifolia and P. kingii subsp. kaibabensis. The latter subspecies, the Kaibab bladderpod, is rare and endemic to the Kaibab Plateau and is a potential candidate to be listed under the Endangered Species Act. Morphological characters, primarily flower color, have been used to distinguish P. kingii subsp. kaibabensis from other subspecies. Here we aim to assess its genetic diversity and differentiation as compared to congeners on and around the Kaibab Plateau. We genotyped DNA obtained from leaf samples from 463 individuals collected from 26 sites representing the three putative taxa (12 P. kingii subsp. kaibabensis, 8 P. kingii subsp. latifolia, and 6 P. arizonica). Our results showed that all samples initially identified as P. kingii subsp. latifolia and P. kingii subsp. kaibabensis on the Kaibab Plateau form a single genetic cluster that is well-differentiated from P. kingii subsp. latifolia sampled from sites off the plateau or P. arizonica on or off the plateau. For P. kingii on the plateau, our findings do not support the previous subspecies designations based on morphological characters. While additional studies of P. kingii will further resolve taxonomic uncertainties within this species, our findings indicate that the Kaibab Plateau population is genetically diverse and genetically distinct, and federal protection is justified in light of the threats faced on the Kaibab Plateau and its limited range.
KW - conservation
KW - genetic diversity
KW - Kaibab Plateau
KW - Physaria
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UR - http://www.scopus.com/inward/citedby.url?scp=85209641091&partnerID=8YFLogxK
U2 - 10.1002/ece3.70523
DO - 10.1002/ece3.70523
M3 - Article
C2 - 39559472
AN - SCOPUS:85209641091
SN - 2045-7758
VL - 14
JO - Ecology and Evolution
JF - Ecology and Evolution
IS - 11
M1 - e70523
ER -