Genetic differentiation among Maruca vitrata F. (Lepidoptera: Crambidae) populations on cultivated cowpea and wild host plants: Implications for insect resistance management and biological control strategies

Tolulope A. Agunbiade, Brad S. Coates, Benjamin Datinon, Rousseau Djouaka, Weilin Sun, Manuele Tamò, Barry R. Pittendrigh

Research output: Contribution to journalArticlepeer-review

Abstract

Maruca vitrata Fabricius (Lepidoptera: Crambidae) is a polyphagous insect pest that feeds on a variety of leguminous plants in the tropics and subtropics. The contribution of host-associated genetic variation on population structure was investigated using analysis of mitochondrial cytochrome oxidase 1 (cox1) sequence and microsatellite marker data from M. vitrata collected from cultivated cowpea (Vigna unguiculata L. Walp.), and alternative host plants Pueraria phaseoloides (Roxb.) Benth. var. javanica (Benth.) Baker, Loncocarpus sericeus (Poir), and Tephrosia candida (Roxb.). Analyses of microsatellite data revealed a significant global FST estimate of 0.05 (P≤0.001). The program STRUCTURE estimated 2 genotypic clusters (co-ancestries) on the four host plants across 3 geographic locations, but little geographic variation was predicted among genotypes from different geographic locations using analysis of molecular variance (AMOVA; among group variation -0.68%) or F-statistics (F STLoc = -0.01; P = 0.62). These results were corroborated by mitochondrial haplotype data (φSTLoc = 0.05; P = 0.92). In contrast, genotypes obtained from different host plants showed low but significant levels of genetic variation (FSTHost = 0.04; P = 0.01), which accounted for 4.08% of the total genetic variation, but was not congruent with mitochondrial haplotype analyses (φST Host = 0.06; P = 0.27). Variation among host plants at a location and host plants among locations showed no consistent evidence for M. vitrata population subdivision. These results suggest that host plants do not significantly influence the genetic structure of M. vitrata, and this has implications for biocontrol agent releases as well as insecticide resistance management (IRM) for M. vitrata in West Africa.

Original languageEnglish (US)
Article numbere92072
JournalPloS one
Volume9
Issue number3
DOIs
StatePublished - Mar 19 2014

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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