Genetic differences between blight-causing Erwinia species with differing host specificities, identified by suppression subtractive hybridization

Lindsay R. Triplett, Youfu Zhao, George W. Sundin

Research output: Contribution to journalArticlepeer-review

Abstract

PCR-based subtractive hybridization was used to isolate sequences from Erwinia amylovora strain Ea110, which is pathogenic on apples and pears, that were not present in three closely related strains with differing host specificities: E. amylavora MR1, which is pathogenic only on Riibus spp.; Erwinia pyrifoliae Ep1/96, the causal agent of shoot blight of Asian pears; and Erwinia sp. strain Ejp556, the causal agent of bacterial shoot blight of pear in Japan. In total, six subtractive libraries were constructed and analyzed. Recovered sequences included type III secretion components, hypothetical membrane proteins, and ATP-binding proteins. In addition, we identified an Ea110-specific sequence with homology to a type III secretion apparatus component of the insect endosymbiont Sodalis glossinidius, as well as an Ep1/96-specific sequence with homology to the Yersinia pestis effector protein tyrosine phosphatase YopH.

Original languageEnglish (US)
Pages (from-to)7359-7364
Number of pages6
JournalApplied and environmental microbiology
Volume72
Issue number11
DOIs
StatePublished - Nov 2006

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Fingerprint

Dive into the research topics of 'Genetic differences between blight-causing Erwinia species with differing host specificities, identified by suppression subtractive hybridization'. Together they form a unique fingerprint.

Cite this