TY - JOUR
T1 - Genetic differences between blight-causing Erwinia species with differing host specificities, identified by suppression subtractive hybridization
AU - Triplett, Lindsay R.
AU - Zhao, Youfu
AU - Sundin, George W.
PY - 2006/11
Y1 - 2006/11
N2 - PCR-based subtractive hybridization was used to isolate sequences from Erwinia amylovora strain Ea110, which is pathogenic on apples and pears, that were not present in three closely related strains with differing host specificities: E. amylavora MR1, which is pathogenic only on Riibus spp.; Erwinia pyrifoliae Ep1/96, the causal agent of shoot blight of Asian pears; and Erwinia sp. strain Ejp556, the causal agent of bacterial shoot blight of pear in Japan. In total, six subtractive libraries were constructed and analyzed. Recovered sequences included type III secretion components, hypothetical membrane proteins, and ATP-binding proteins. In addition, we identified an Ea110-specific sequence with homology to a type III secretion apparatus component of the insect endosymbiont Sodalis glossinidius, as well as an Ep1/96-specific sequence with homology to the Yersinia pestis effector protein tyrosine phosphatase YopH.
AB - PCR-based subtractive hybridization was used to isolate sequences from Erwinia amylovora strain Ea110, which is pathogenic on apples and pears, that were not present in three closely related strains with differing host specificities: E. amylavora MR1, which is pathogenic only on Riibus spp.; Erwinia pyrifoliae Ep1/96, the causal agent of shoot blight of Asian pears; and Erwinia sp. strain Ejp556, the causal agent of bacterial shoot blight of pear in Japan. In total, six subtractive libraries were constructed and analyzed. Recovered sequences included type III secretion components, hypothetical membrane proteins, and ATP-binding proteins. In addition, we identified an Ea110-specific sequence with homology to a type III secretion apparatus component of the insect endosymbiont Sodalis glossinidius, as well as an Ep1/96-specific sequence with homology to the Yersinia pestis effector protein tyrosine phosphatase YopH.
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U2 - 10.1128/AEM.01159-06
DO - 10.1128/AEM.01159-06
M3 - Article
C2 - 16963554
AN - SCOPUS:33751007012
SN - 0099-2240
VL - 72
SP - 7359
EP - 7364
JO - Applied and environmental microbiology
JF - Applied and environmental microbiology
IS - 11
ER -