Genetic characterization of the Escherichia coli cyclopropane fatty acid (cfa) locus and neighboring loci

Dennis W. Grogan, John E. Cronan

Research output: Contribution to journalArticlepeer-review

Abstract

The phenotypically silent cyclopropane fatty acid synthesis (cfa) gene of Escherichia coli K-12 has been located on the genetic linkage map. This was accomplished by integrating (via homologous recombination) the selectable marker of a recombinant plasmid into the host chromosome near the cfa locus. This integration allowed the subsequent isolation of a cfa-linked transposon Tn 10 insertion. Genetic mapping of the Tn 10 insertion, using conventional techniques, placed the cfa locus at min 36.5 on the linkage map in the vicinity of several other non-selectable markers. We ordered cfa and these other loci by three-factor transductional analyses. Selection for excision of the Tn 10 element resulted in several types of mutants which harbor mutations of cfa and of neighboring genes, presumably as a consequence of Tn 10-catalyzed chromosomal rearrangements.

Original languageEnglish (US)
Pages (from-to)367-372
Number of pages6
JournalMGG Molecular & General Genetics
Volume196
Issue number2
DOIs
StatePublished - Sep 1984

ASJC Scopus subject areas

  • Genetics

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