TY - JOUR
T1 - Genetic characterization and recombination analysis of atypical porcine pestivirus
AU - Guo, Zhenhua
AU - Wang, Leyi
AU - Qiao, Songlin
AU - Deng, Ruiguang
AU - Zhang, Gaiping
N1 - Funding Information:
This study was performed according to the recommendations of National Standards for Laboratory Animals of the People's Republic of China (GB149258-2010). Ethics approval was given by the Animal Experiments Committee of the Henan Provincial Key Laboratory of Animal Immunology.This work was funded by grants from the National Key Research and Development Program of China (2016YFD0500709), the National Natural Science Foundation of China (31902281), China Agriculture Research System (CARS-35) and the Special Fund for Henan Agriculture Research System (S2012-06).
Funding Information:
This work was funded by grants from the National Key Research and Development Program of China ( 2016YFD0500709 ), the National Natural Science Foundation of China ( 31902281 ), China Agriculture Research System ( CARS-35 ) and the Special Fund for Henan Agriculture Research System ( S2012-06 ).
PY - 2020/7
Y1 - 2020/7
N2 - Atypical porcine pestivirus (APPV) is recognised as the etiology of congenital tremor (CT) Type A-II and poses a challenge to pig production. Here, we described a CT case in piglets caused by APPV infection in central China in 2017. Interestingly, different from a previous report, more CT litters were observed in the second and third parity sows compared to the first and fourth parity. Evolutionary analysis and recombination evaluation were conducted for the isolate and 61 APPV genomes were available in GenBank. Phylogenetic analysis revealed a high level of genetic variation of APPV and the coexistence of three clades (Clades I–III) in China. The isolate was clustered into Clade I, which seemed to be prevalent worldwide and displayed higher genetic variability (Subgroups 1–4) compared with Clade II and Clade III, both of which were only reported in China. Notably, three putative recombinants were identified and characterized in APPV. The recombination events occurred in inter-clades (Clade II and III) or intra-clades (Clade I). To the best of our knowledge, this study presents the first evidence of homologous recombination within Pestivirus K. These results provide new clinical presentations of APPV infection and may be helpful in better understanding the large amount of genetic variations in this genus.
AB - Atypical porcine pestivirus (APPV) is recognised as the etiology of congenital tremor (CT) Type A-II and poses a challenge to pig production. Here, we described a CT case in piglets caused by APPV infection in central China in 2017. Interestingly, different from a previous report, more CT litters were observed in the second and third parity sows compared to the first and fourth parity. Evolutionary analysis and recombination evaluation were conducted for the isolate and 61 APPV genomes were available in GenBank. Phylogenetic analysis revealed a high level of genetic variation of APPV and the coexistence of three clades (Clades I–III) in China. The isolate was clustered into Clade I, which seemed to be prevalent worldwide and displayed higher genetic variability (Subgroups 1–4) compared with Clade II and Clade III, both of which were only reported in China. Notably, three putative recombinants were identified and characterized in APPV. The recombination events occurred in inter-clades (Clade II and III) or intra-clades (Clade I). To the best of our knowledge, this study presents the first evidence of homologous recombination within Pestivirus K. These results provide new clinical presentations of APPV infection and may be helpful in better understanding the large amount of genetic variations in this genus.
KW - Atypical porcine pestivirus
KW - Congenital tremor
KW - Phylogeny
KW - Recombination
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U2 - 10.1016/j.meegid.2020.104259
DO - 10.1016/j.meegid.2020.104259
M3 - Article
C2 - 32087344
AN - SCOPUS:85080071613
VL - 81
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
SN - 1567-1348
M1 - 104259
ER -