Genetic and biochemical analyses of Escherichia coli strains having a mutation in the structural gene (poxB) for pyruvate oxidase

Ying Chang Ying Ying Chang, John E Cronan

Research output: Contribution to journalArticle

Abstract

Mutants of Escherichia coli K-12 deficient in pyruvate oxidase were isolated from an aceEF (pyruvate dehydrogenase-deficient) strain by selection for a complete absence of growth on medium lacking acetate. Extracts of two of the mutants were shown to contain normal levels of pyrivate oxidase antigen, although the enzymatic activities of the extracts were reduced or absent. The poxB locus was mapped by using closely linked transposon insertions to min 18.7 of the E. coli linkage map between the cmlA and aroA loci, a location far removed from that of the regulatory gene, poxA.

Original languageEnglish (US)
Pages (from-to)756-762
Number of pages7
JournalJournal of Bacteriology
Volume154
Issue number2
StatePublished - 1983

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

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