TY - JOUR
T1 - Genes and proteins associated with ribeye area and meat tenderness in a commercial Nellore cattle population
AU - Frezarim, Gabriela B.
AU - Fonseca, Larissa F.S.
AU - Salatta, Bruna M.
AU - Silva, Danielly B.S.
AU - Bresolin, Tiago
AU - Oliveira Seno, Leonardo de
AU - Barufatti, Alexéia
AU - Ferro, Jesus Aparecido
AU - Albuquerque, Lucia Galvão
PY - 2022/4/1
Y1 - 2022/4/1
N2 - Despite several studies on genetic markers and differentially expressed genes related to ribeye area (REA) and tenderness traits in beef cattle, there is divergence in the results regarding the genes associated with these traits. Thirteen genes associated with or exhibiting biological functions that might influence such phenotypes were included in this study. A total of five genes for REA (IGF-1, IGF-2, MSTN, NEDD4, and UBE4A) and eight genes for meat tenderness (CAPN1, CAPN2, CAST, HSPB1, DNAJA1, FABP4, SCD, and PRKAG3) were selected from previous studies on beef cattle. Genes and their respective proteins expression were validated in a commercial population of Nellore cattle using quantitative real-time PCR (RT-qPCR) and advanced mass spectrometry (LC/MS-MS) techniques, respectively. The MSTN gene was upregulated in animals with low REA. The CAPN1, CAPN2, CAST, HSPB1, and DNAJA1 genes were upregulated in animals with tough meat. The proteins translated by these genes were not differentially expressed. Our results confirm the potential of some of the studied genes as biomarkers for carcass and meat quality traits in Nellore cattle.
AB - Despite several studies on genetic markers and differentially expressed genes related to ribeye area (REA) and tenderness traits in beef cattle, there is divergence in the results regarding the genes associated with these traits. Thirteen genes associated with or exhibiting biological functions that might influence such phenotypes were included in this study. A total of five genes for REA (IGF-1, IGF-2, MSTN, NEDD4, and UBE4A) and eight genes for meat tenderness (CAPN1, CAPN2, CAST, HSPB1, DNAJA1, FABP4, SCD, and PRKAG3) were selected from previous studies on beef cattle. Genes and their respective proteins expression were validated in a commercial population of Nellore cattle using quantitative real-time PCR (RT-qPCR) and advanced mass spectrometry (LC/MS-MS) techniques, respectively. The MSTN gene was upregulated in animals with low REA. The CAPN1, CAPN2, CAST, HSPB1, and DNAJA1 genes were upregulated in animals with tough meat. The proteins translated by these genes were not differentially expressed. Our results confirm the potential of some of the studied genes as biomarkers for carcass and meat quality traits in Nellore cattle.
KW - beef cattle
KW - biological processes
KW - bovins de boucherie
KW - expression génique
KW - gene expression
KW - meat quality
KW - processus biologiques
KW - qualité de la viande
KW - zebu
KW - zébu
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UR - http://www.scopus.com/inward/citedby.url?scp=85127476142&partnerID=8YFLogxK
U2 - 10.1139/gen-2020-0163
DO - 10.1139/gen-2020-0163
M3 - Article
C2 - 34860606
AN - SCOPUS:85127476142
SN - 0831-2796
VL - 65
SP - 229
EP - 240
JO - Genome
JF - Genome
IS - 4
ER -