Generation of transgenic dogs that conditionally express green fluorescent protein

Min Jung Kim, Hyun Ju Oh, Jung Eun Park, Geon A. Kim, So Gun Hong, Goo Jang, Mo Sun Kwon, Bon Chul Koo, Teoan Kim, Sung Keun Kang, Jeong Chan Ra, Chemyong Ko, Byeong Chun Lee

Research output: Contribution to journalArticle

Abstract

We report the creation of a transgenic dog that conditionally expresses eGFP (enhanced green fluorescent protein) under the regulation of doxycycline. Briefly, fetal fibroblasts infected with a Tet-on eGFP vector were used for somatic cell nuclear transfer. Subsequently reconstructed oocytes were transferred to recipients. Three clones having transgenes were born and one dog was alive. The dog showed all features of inducible expression of eGFP upon doxycycline administration, and successful breeding resulted in eGFP-positive puppies, confirming stable insertion of the transgene into the genome. This inducible dog model will be useful for a variety of medical research studies.

Original languageEnglish (US)
Pages (from-to)472-478
Number of pages7
JournalGenesis
Volume49
Issue number6
DOIs
StatePublished - Jun 2011

Keywords

  • Dog
  • Somatic cell nuclear transfer
  • Tetracycline vector
  • Transgenic

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

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