Generation of transgenic dogs that conditionally express green fluorescent protein

Min Jung Kim, Hyun Ju Oh, Jung Eun Park, Geon A. Kim, So Gun Hong, Goo Jang, Mo Sun Kwon, Bon Chul Koo, Teoan Kim, Sung Keun Kang, Jeong Chan Ra, Chemyong Ko, Byeong Chun Lee

Research output: Contribution to journalArticle

Abstract

We report the creation of a transgenic dog that conditionally expresses eGFP (enhanced green fluorescent protein) under the regulation of doxycycline. Briefly, fetal fibroblasts infected with a Tet-on eGFP vector were used for somatic cell nuclear transfer. Subsequently reconstructed oocytes were transferred to recipients. Three clones having transgenes were born and one dog was alive. The dog showed all features of inducible expression of eGFP upon doxycycline administration, and successful breeding resulted in eGFP-positive puppies, confirming stable insertion of the transgene into the genome. This inducible dog model will be useful for a variety of medical research studies.

Original languageEnglish (US)
Pages (from-to)472-478
Number of pages7
JournalGenesis
Volume49
Issue number6
DOIs
StatePublished - Jun 1 2011
Externally publishedYes

Fingerprint

Green Fluorescent Proteins
Dogs
Doxycycline
Transgenes
Oocytes
Breeding
Biomedical Research
Clone Cells
Fibroblasts
Genome
enhanced green fluorescent protein

Keywords

  • Dog
  • Somatic cell nuclear transfer
  • Tetracycline vector
  • Transgenic

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

Cite this

Kim, M. J., Oh, H. J., Park, J. E., Kim, G. A., Hong, S. G., Jang, G., ... Lee, B. C. (2011). Generation of transgenic dogs that conditionally express green fluorescent protein. Genesis, 49(6), 472-478. https://doi.org/10.1002/dvg.20737

Generation of transgenic dogs that conditionally express green fluorescent protein. / Kim, Min Jung; Oh, Hyun Ju; Park, Jung Eun; Kim, Geon A.; Hong, So Gun; Jang, Goo; Kwon, Mo Sun; Koo, Bon Chul; Kim, Teoan; Kang, Sung Keun; Ra, Jeong Chan; Ko, Chemyong; Lee, Byeong Chun.

In: Genesis, Vol. 49, No. 6, 01.06.2011, p. 472-478.

Research output: Contribution to journalArticle

Kim, MJ, Oh, HJ, Park, JE, Kim, GA, Hong, SG, Jang, G, Kwon, MS, Koo, BC, Kim, T, Kang, SK, Ra, JC, Ko, C & Lee, BC 2011, 'Generation of transgenic dogs that conditionally express green fluorescent protein', Genesis, vol. 49, no. 6, pp. 472-478. https://doi.org/10.1002/dvg.20737
Kim MJ, Oh HJ, Park JE, Kim GA, Hong SG, Jang G et al. Generation of transgenic dogs that conditionally express green fluorescent protein. Genesis. 2011 Jun 1;49(6):472-478. https://doi.org/10.1002/dvg.20737
Kim, Min Jung ; Oh, Hyun Ju ; Park, Jung Eun ; Kim, Geon A. ; Hong, So Gun ; Jang, Goo ; Kwon, Mo Sun ; Koo, Bon Chul ; Kim, Teoan ; Kang, Sung Keun ; Ra, Jeong Chan ; Ko, Chemyong ; Lee, Byeong Chun. / Generation of transgenic dogs that conditionally express green fluorescent protein. In: Genesis. 2011 ; Vol. 49, No. 6. pp. 472-478.
@article{0a5f3d0081a44ac3bd9c6c044e17691b,
title = "Generation of transgenic dogs that conditionally express green fluorescent protein",
abstract = "We report the creation of a transgenic dog that conditionally expresses eGFP (enhanced green fluorescent protein) under the regulation of doxycycline. Briefly, fetal fibroblasts infected with a Tet-on eGFP vector were used for somatic cell nuclear transfer. Subsequently reconstructed oocytes were transferred to recipients. Three clones having transgenes were born and one dog was alive. The dog showed all features of inducible expression of eGFP upon doxycycline administration, and successful breeding resulted in eGFP-positive puppies, confirming stable insertion of the transgene into the genome. This inducible dog model will be useful for a variety of medical research studies.",
keywords = "Dog, Somatic cell nuclear transfer, Tetracycline vector, Transgenic",
author = "Kim, {Min Jung} and Oh, {Hyun Ju} and Park, {Jung Eun} and Kim, {Geon A.} and Hong, {So Gun} and Goo Jang and Kwon, {Mo Sun} and Koo, {Bon Chul} and Teoan Kim and Kang, {Sung Keun} and Ra, {Jeong Chan} and Chemyong Ko and Lee, {Byeong Chun}",
year = "2011",
month = "6",
day = "1",
doi = "10.1002/dvg.20737",
language = "English (US)",
volume = "49",
pages = "472--478",
journal = "Genesis",
issn = "1526-954X",
publisher = "Wiley-Liss Inc.",
number = "6",

}

TY - JOUR

T1 - Generation of transgenic dogs that conditionally express green fluorescent protein

AU - Kim, Min Jung

AU - Oh, Hyun Ju

AU - Park, Jung Eun

AU - Kim, Geon A.

AU - Hong, So Gun

AU - Jang, Goo

AU - Kwon, Mo Sun

AU - Koo, Bon Chul

AU - Kim, Teoan

AU - Kang, Sung Keun

AU - Ra, Jeong Chan

AU - Ko, Chemyong

AU - Lee, Byeong Chun

PY - 2011/6/1

Y1 - 2011/6/1

N2 - We report the creation of a transgenic dog that conditionally expresses eGFP (enhanced green fluorescent protein) under the regulation of doxycycline. Briefly, fetal fibroblasts infected with a Tet-on eGFP vector were used for somatic cell nuclear transfer. Subsequently reconstructed oocytes were transferred to recipients. Three clones having transgenes were born and one dog was alive. The dog showed all features of inducible expression of eGFP upon doxycycline administration, and successful breeding resulted in eGFP-positive puppies, confirming stable insertion of the transgene into the genome. This inducible dog model will be useful for a variety of medical research studies.

AB - We report the creation of a transgenic dog that conditionally expresses eGFP (enhanced green fluorescent protein) under the regulation of doxycycline. Briefly, fetal fibroblasts infected with a Tet-on eGFP vector were used for somatic cell nuclear transfer. Subsequently reconstructed oocytes were transferred to recipients. Three clones having transgenes were born and one dog was alive. The dog showed all features of inducible expression of eGFP upon doxycycline administration, and successful breeding resulted in eGFP-positive puppies, confirming stable insertion of the transgene into the genome. This inducible dog model will be useful for a variety of medical research studies.

KW - Dog

KW - Somatic cell nuclear transfer

KW - Tetracycline vector

KW - Transgenic

UR - http://www.scopus.com/inward/record.url?scp=79958807684&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79958807684&partnerID=8YFLogxK

U2 - 10.1002/dvg.20737

DO - 10.1002/dvg.20737

M3 - Article

C2 - 21630415

AN - SCOPUS:79958807684

VL - 49

SP - 472

EP - 478

JO - Genesis

JF - Genesis

SN - 1526-954X

IS - 6

ER -