Generation of red fluorescent protein transgenic dogs

So Gun Hong, Min Kyu Kim, Goo Jang, Hyun Ju Oh, Jung Eun Park, Jung Taek Kang, Ok Jae Koo, Teoan Kim, Mo Sun Kwon, Bon Chul Koo, Jeong Chan Ra, Dae Yong Kim, Che Myong Ko, Byeong Chun Lee

Research output: Contribution to journalArticlepeer-review

Abstract

Dogs (Canis familiaris) share many common genetic diseases with humans and development of disease models using a transgenic approach has long been awaited. However, due to the technical difficulty in obtaining fertilizable eggs and the unavailability of embryonic stem cells, no transgenic dog has been generated. Canine fetal fibroblasts were stably transfected with a red fluorescent protein (RFP) gene-expressing construct using retrovirus gene delivery method. Somatic cell nuclear transfer was then employed to replace the nucleus of an oocyte with the nucleus of the RFP-fibroblasts. Using this approach, we produced the first generation of transgenic dogs with four female and two male expressing RFP.

Original languageEnglish (US)
Pages (from-to)314-322
Number of pages9
JournalGenesis
Volume47
Issue number5
DOIs
StatePublished - 2009
Externally publishedYes

Keywords

  • Dog
  • Fetal fibroblasts
  • Nuclear transfer
  • Red fluorescent protein
  • Transgenic

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Generation of red fluorescent protein transgenic dogs'. Together they form a unique fingerprint.

Cite this